1BR.3.GN

90020509, human skin, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: 1BR.3.GN, 90020509
• Origine biologica: human skin
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human skin fibroblast, transformed

Descrizione della linea cellulare

Derived from a normal fibroblast (1BR3 Sigma Catalogue number. 90011801) transformed with the plasmids pSV3gpt and pSV3neo. The cells were selected for pSV2neo only.The genetic identity of 1BR3 (90011801), 1BR.3.G (90020507), 1BR.3.GN (90020508), and 1BR.3.N (90020509) has been confirmed by STR profiling at ECACC.

Applicazioni

DNA repair and cell growth studies

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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