HEL 299

87042207, human lung (embryonic), Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: HEL 299, 87042207
• Origine biologica: human lung (embryonic)
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 46, diploid
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human embryo lung

Descrizione della linea cellulare

Derived from embryonic lung tissue of a male in 1965. It is one of the few human diploid lines to have type A glucose-6-phosphate dehydrogenase. Cells have a limited lifespan of 20 serial subcultures from tissue of origin. Ethnicity: Black.

Applicazioni

HEL 299 has been used to study the antiviral properties of AIC246, a novel anti-human cytomegalovirus compound. It has also been used to study the role of SUMO ligase protein inhibitor of activated STAT1 (PIAS1) in the intrinsic antiviral immune response to herpes simplex virus.

Profilo DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 11,12
D16S539: 10,11
D5S818: 11,13
D7S820: 8,11
THO1: 7
TPOX: 8,12
vWA: 16

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Limited lifespan.

Altre note

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