EIII

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: Human x Mouse Hybridoma hybrid
• Confezionamento: tube of 5 μg 86072804-DNA-5UG; pkg of vial of cells 86072804-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: pJ3.11, p79a, CFTR DNA markers
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human x mouse hybrid

Descrizione della linea cellulare

This cell line may contain a sub-chromosomal fragment of human chromosome 17 bearing markers thought at the time to flank the cytotic fibrosis gene. The human DNA was donated from the human osteosarcoma cell line MNNG-HOS.

Applicazioni

Somatic cell hybrid mapping

Terreno di coltura

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO₂.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.