C-14030

Normal Human Dermal Fibroblasts (NHDF)

Juvenile foreskin, 1 million cells per pellet

• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human foreskin (juvenile dermis)
• Morphology: (fibroblast)

Proprietà

• biological source: human foreskin (juvenile dermis)
• packaging: pkg of 1,000,000 cells
• morphology: (fibroblast)
• application(s): cell analysis (DNA, RNA, and protein)
• shipped in: dry ice
• storage temp.: −20°C

Descrizione

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.

Application

Primary Normal Human Dermal Fibroblasts (NHDF) are isolated from the dermis of juvenile foreskin. They can be used for wound healing studies and dermatological research to investigate diseases like scleroderma, fibrosarcoma, fibrosis, xeroderma pigmentosum, and histiocytoma. Moreover, fibroblasts are important for cancer research, tissue regeneration, and tissue engineering studies.Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.

Biochem/physiol Actions

Skin

Physical form

Pellet is dissolved in RNAlater^® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Preparation Note

Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Primary Cell Culture Media:Link

Recommended Primary Cell Culture Media:Link

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Legal Information

RNAlater is a registered trademark of Thermo Fisher Scientific, Inc.

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

Informazioni sulla sicurezza

• Classe di stoccaggio: 12 - Non Combustible Liquids
• WGK: WGK 1
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable