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C-12750

Sigma-Aldrich

Human Chondrocytes (HCH)

500,000 proliferating cells

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About This Item

Codice UNSPSC:
41106514
NACRES:
NA.81

Origine biologica

human articular cartilage (knee and hip joints)

Confezionamento

pkg of 500,000 cells

Morfologia

(chondrocytes)

tecniche

cell culture | mammalian: suitable

Condizioni di spedizione

ambient

Temperatura di conservazione

room temp

Descrizione generale

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Origine della linea cellulare

Cartilage

Applicazioni

Primary Human Chondrocytes (HCH) are isolated from normal human articular cartilage from the knee and hip joints (lot specific source information is available on request) and are provided in a proliferating format. PromoCell HCH are routinely tested for their capacity to differentiate in 3D spheroids.The articular cartilage covers the joints between bones and contains no blood vessels, lymphatic vessels, or nerve fibers. It is composed of only one specialized cell type, the chondrocytes, which produce and maintain the extracellular matrix of cartilage, i.e. collagen (mostly type II) and proteoglycans (primarily aggrecan).

Qualità

Rigid quality control tests are performed for each lot of Human Chondrocytes. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, each lot is routinely tested for the capacity to differentiate in 3D spheroids. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV and HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).

Attenzione

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Mantenimento delle subcolture

Click here for more information.

Altre note

Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: Differentiation testedGuaranteed population doublings: >10

Prodotti consigliati

Recommended Primary Cell Culture Media:Link

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Articoli

Primary human chondrocyte cells and optimized growth media for the in vitro cultivation of chondrocytes. Protocols for handling, cell passaging, media, and product use.

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