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Key Documents

C-12252

Sigma-Aldrich

Human Umbilical Artery Endothelial Cells (HUAEC)

500,000 proliferating cells

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About This Item

Codice UNSPSC:
41106514
NACRES:
NA.81

Origine biologica

human umbilical cord artery

Confezionamento

pkg of 500,000 cells

Morfologia

( endothelial)

tecniche

cell culture | mammalian: suitable

Malattie correlate

cardiovascular diseases

Condizioni di spedizione

ambient

Temperatura di conservazione

room temp

Descrizione generale

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Origine della linea cellulare

Artery

Applicazioni

Primary Human Umbilical Artery Endothelial Cells (HUAEC) are isolated from the arteries of the umbilical cord and are provided in a proliferating format. They are often used for in vitro studies on atherosclerosis but also for endothelial cell-dependent lymphocyte activation and chemoattractant activity experiments. (Human Umbilical Vein Endothelial Cells (HUVEC) from the same donor are available on request).

Qualità

Rigid quality control tests are performed for each lot of Endothelial Cells from large vessels. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. von Willebrand Factor (vWF) and CD31, as well as Dil-Ac-LDL uptake assays are also carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Attenzione

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Mantenimento delle subcolture

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Altre note

Recommended Plating Density: 5000 - 10000 cells per cm2Passage After Thawing: P1Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Prodotti consigliati

Recommended Primary Cell Culture Media:Link

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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