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C-12071

Sigma-Aldrich

Human Follicle Dermal Papilla Cells (HFDPC)

500,000 cryopreserved cells

Sinonimo/i:

HFDPC cells

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About This Item

Codice UNSPSC:
41106514
NACRES:
NA.81

Origine biologica

human dermis

Confezionamento

pkg of 500,000 cells

Morfologia

( dermal)

tecniche

cell culture | mammalian: suitable

Condizioni di spedizione

dry ice

Temperatura di conservazione

−196°C

Categorie correlate

Descrizione generale

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Origine della linea cellulare

Skin

Applicazioni

Primary Human Follicle Dermal Papilla Cells (HFDPC) are isolated mainly from human dermis of the scalp, originating from the occipital or temple region and are provided in a cryopreserved format. Information on donor hair and skin color is available on request. HFDPC stain positive for alkaline phosphatase.Dermal papillae are embedded in a laminin and collagen IV rich extracellular matrix at the base of the hair follicles. They are essential for the induction and maintenance of hair growth. Since they have androgen receptors, they can be used for in vitro screening of androgen blocking reagents.

Qualità

Rigid quality control tests are performed for each lot of Follicle Dermal Papilla Cells. They are tested for cell morphology, adherence rate, and cell viability. Immunohistochemical tests for significant markers, e.g. alkaline phosphatase, are carried out for each lot. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).

Attenzione

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Mantenimento delle subcolture

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Altre note

Recommended Plating Density: 5000 - 10000 cells per cm2Passage After Thawing: P2Tested Markers: Alkaline phosphatase positiveGuaranteed population doublings: >10

Prodotti consigliati

Recommended Primary Cell Culture Media:Link

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Articoli

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Yeast plasmid selection: Utilizes auxotrophic yeast strains for plasmid selection, enabling growth on synthetic media for better yield.

Overview of primary cells and media usage in biomedical culture applications highlights differences from cell lines.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

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