A4487

Aphidicolin, Ready Made Solution

from Nigrospora sphaerica

• Sinonimo/i: ICI 69653, NSC-234714
• Formula empirica (notazione di Hill): C₂₀H₃₄O₄
• Numero CAS: 38966-21-1
• Peso molecolare: 338.48
• Numero CE: 200-664-3
• Numero MDL: MFCD00083214
• Codice UNSPSC: 51102829
• NACRES: NA.85

Proprietà

• Origine biologica: Nigrospora sphaerica
• Livello qualitativo: 200
• Tensione di vapore: 0.55 hPa ( 20 °C)
• Stato: liquid
• Colore: clear colorless
• Solubilità: H₂O: miscible (completely)
• Spettro attività antibiotica: neoplastics; viruses
• Modalità d’azione: DNA synthesis | interferes; enzyme | inhibits
• Temperatura di conservazione: −20°C
• Stringa SMILE: O[C@]1([C@H]2C[C@@]3([C@@]4([C@H]([C@@]([C@@H](CC4)O)(CO)C)CC[C@H]3C2)C)CC1)CO
• InChI: 1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1; NOFOAYPPHIUXJR-APNQCZIXSA-N

Descrizione

Descrizione generale

Aphidicolin is a tetracyclic diterpene with antiviral and antimitotic properties used for cell cycle synchronization in various cell lines.[1]

Applicazioni

Aphidicolin, from Nigrospora sphaerica, was used for cell cycle synchronization of tobacco BY-2 cells. It is used to block the entry of cells into S-phase.[2]

Azioni biochim/fisiol

Aphidicolin specifically inhibits DNA polymerase α that is responsible for DNA replication. It also inhibits α-like DNA polymerases of plants and yeasts but does not inhibit synthesis of RNA and proteins. Aphidicolin competes for the dCTP-specific binding site on DNA polymerase α specifically. Aphidicolin has antiviral and antimitotic properties. It blocks the cell cycle at S phase. It has been shown to induce apoptosis in HeLa cells.[3]

Confezionamento

1 ml

Stato fisico

Aphidicolin Ready Made Solution supplied as a 1 mg/ml solution in DMSO.

Altre note

Keep container tightly closed in a dry and well-ventilated place. Storage class (TRGS 510): Combustible liquids

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): 188.6 °F - closed cup
• Punto d’infiammabilità (°C): 87 °C - closed cup