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A4058

Sigma-Aldrich

Acrylamide solution

40%, suitable for electrophoresis, sterile-filtered

Sinonimo/i:

2-Propenamide, 40% Acrylamido solution, Acrylic amide, Akrylamid, Polyacrylamide solution

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100 ML
113,00 €
5 L
1.050,00 €

113,00 €


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Cambia visualizzazione
100 ML
113,00 €
5 L
1.050,00 €

About This Item

Formula condensata:
CH2=CHCONH2
Numero CAS:
Peso molecolare:
71.08
Beilstein:
6185892
Numero MDL:
Codice UNSPSC:
41105319
eCl@ss:
39031205
ID PubChem:
NACRES:
NB.22

113,00 €


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Sterilità

sterile-filtered

Stato

liquid

Concentrazione

40%

tecniche

electrophoresis: suitable

Temperatura di conservazione

2-8°C

Stringa SMILE

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)
HRPVXLWXLXDGHG-UHFFFAOYSA-N

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Descrizione generale

Acrylamide has been classified as probably carcinogenic in humans. It is formed in fried and oven-cooked foods due to Maillard reaction between amino acids and reducing sugars.[1]

Applicazioni

Acrylamide solution is suitable for use in electrophoresis. It may be used in the preparation of gel buffer for the determination of molecular weight of polypeptide chains by polyacrylamide gel electrophoresis.[2] It may be used in the electrochemical polymerization of pyrrole.[3] It may be used to prepare the pH 7.6 gels, required for the determination of molecular weights of histone by sodium dodecyl sulfate gel electrophoresis.[4]

Nota sulla preparazione

Prepared from electrophoresis grade acrylamide in ultra-pure water.

Pittogrammi

Health hazardExclamation mark

Avvertenze

Danger

Classi di pericolo

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Organi bersaglio

Peripheral nervous system

Codice della classe di stoccaggio

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Elenchi normativi

Forniamo informazioni su eventuali restrizioni prevalentemente per i prodotti chimici. Per altre tipologie di prodotto siamo in grado di fornire soltanto informazioni limitate. Nessuna segnalazione significa che nessuno dei componenti è citato in un elenco. È dovere dell’utilizzatore assicurarsi che il prodotto venga impiegato in maniera sicura e a norme di legge.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificati d'analisi (COA)

Lot/Batch Number

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Electrochemical polymerization of pyrrole in acrylamide solution.
Sarac AS, et al.
Synthetic Metals, 98(3), 177-182 (1999)
The molecular weights of vertebrate histones exploiting a modified sodium dodecyl sulfate electrophoretic method.
S Panyim et al.
The Journal of biological chemistry, 246(24), 7557-7560 (1971-12-25)
Donald S Mottram et al.
Nature, 419(6906), 448-449 (2002-10-09)
Reports of the presence of acrylamide in a range of fried and oven-cooked foods have caused worldwide concern because this compound has been classified as probably carcinogenic in humans. Here we show how acrylamide can be generated from food components
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
K Weber et al.
The Journal of biological chemistry, 244(16), 4406-4412 (1969-08-25)
Arianna Rath et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15668-15673 (2013-09-11)
SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which comprise

Domande

1–4 di 4 domande  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Utile?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Utile?

  3. What is the ratio of acrylamide:bisacrylamide in this product? Is it 37.5:1, 29:1 or 19:1 (or maybe other?)

    1 risposta
    1. This product is simply a 40% solution of Acrylamide and does not contain any Bis-acrylamide. Note that there are several Acrylamide/Bis-acrylamide 40% solutions available. Please see the link below to review these product options:
      https://www.sigmaaldrich.com/substance/acrylamidebisacrylamide40solution1234598765

      Utile?

  4. What is the solvent in this solution. Water? Or organic solvent?

    1 risposta
    1. The solution is prepared from electrophoresis grade acrylamide in ultra-pure water and aseptically filtered to insure the highest quality and reproducibility.

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