This product is Lipase A from Candida antarctica that is covalently attached to an apolar macroporous carrier. This product is not directly screened for solubility. This item is commonly used in aqueous hydrolysis activity assays using 1-10% tributyrin in 25 mM potassium phosphate buffer, pH 7.5 at 40 °C.
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| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
10 g | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 152,00 € |
50 g | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 662,00 € 562,70 € |
Informazioni su questo articolo
NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥500 U/g
Recombinant:
expressed in Aspergillus oryzae
152,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Servizio Tecnico
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Permettici di aiutartirecombinant
expressed in Aspergillus oryzae
Quality Segment
form
beads (powder like), beads
specific activity
≥500 U/g
storage temp.
2-8°C
General description
Research area: Cellsignalling. Lipase A Candida Antarctica, CalA is a thermostable, calcium-dependent enzyme with high substrate specificity. CalA comprises of the catalytic triad (Ser184, Asp334, His366) and has an α/β hydrolase structural fold.
Application
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Lipase A Candida antarctica immobilized on Immobead 150, recombinant from Aspergillus oryzae has been used in the synthesis of enantiopure (R)-salsolinol and adsorption kinetics studies using Quartz crystal microbalance with dissipation (QCM-D).It has also been used tostudy the esterification of difluorinated alcohols.[1]
Biochem/physiol Actions
Lipase A Candida Antarctica, CalA is highly specific for alcohols and esterifies the trans-isomer of fatty acids. CalA recognizes highly branched acyl groups and is active on alcohols with steric hindrance. CalA catalyzes the production of enantiopure amino acids and aids in the synthesis of chiral cyanohydrins. It may find industrial applications for its thermostable functionality in paper industry.Lipase A Candida antarctica(CAL-A) shows acetyltransferase activity by synthesizing fatty acid esters from certain alcohols and natural oils in an aqueous environment.
Other Notes
1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 10.0 and 40°C (tributyrin, Cat. No. 91010, as substrate)
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Questo articolo | |||
|---|---|---|---|
| specific activity ≥500 U/g | specific activity ≥1800 U/g | specific activity ≥2000 U/g | specific activity ~9 U/mg |
| recombinant expressed in Aspergillus oryzae | recombinant expressed in Aspergillus oryzae | recombinant expressed in yeast | recombinant expressed in Aspergillus oryzae |
| form beads (powder like) | form beads | form beads | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the solvent of Lipase A Candida antarctica immobilized on Immobead 150, recombinant from Aspergillus oryzae? What is it soluble in?
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