41348
Anti-Rabbit IgG−Abberior® STAR 635 antibody produced in goat
for STED application
Sinonimo/i:
Abberior® STAR 635-Anti-Rabbit IgG antibody produced in goat
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About This Item
Codice UNSPSC:
12352200
NACRES:
NA.45
Origine biologica
goat
Livello qualitativo
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
secondary antibodies
Clone
polyclonal
Forma fisica
buffered aqueous solution
Reattività contro le specie
rabbit
Concentrazione
~1 mg/mL
Fluorescenza
λex 635 nm; λem 655 nm in PBS, pH 7.4
Temperatura di conservazione
−20°C
Descrizione generale
Abberior STAR 635 is a dye developed for STED and confocal imaging in the red spectral region. It features exceptional photostability, very high brightness and low background labeling. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. Abberior STAR 635 can substitute dyes like Atto™ 647N, Alexa Fluor™ 647, or Cy®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 635 (PBS pH 7.4), 635 (H2O), 635 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 110 000 (PBS pH 7.4), 110 000 (H2O), 115 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,26 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,38 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 655 (PBS pH 7.4), 655 (H2O), 656 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,88 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 2,8 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 635 (PBS pH 7.4), 635 (H2O), 635 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 110 000 (PBS pH 7.4), 110 000 (H2O), 115 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,26 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,38 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 655 (PBS pH 7.4), 655 (H2O), 656 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,88 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 2,8 (PBS pH 7.4)
Applicazioni
Abberior STAR 635 particularly well suits the Abberior Instruments 2-color 775nm STED microscope and the Leica TCS STED system. It delivers high-resolution STED images with very low background signal due to its improved water solubility and exceptional brightness. The dye serves as an ideal partner for several 2-color STED packages.
Compatibilità
Designed and tested for fluorescent super-resolution microscopy
Risultati analitici
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
Altre note
Note legali
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH
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Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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