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12266

Sigma-Aldrich

N-[4-(2-Benzimidazolyl)phenyl]maleimide

BioReagent, suitable for fluorescence, ≥98.0% (HPLC)

Sinonimo/i:

BIPM

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About This Item

Formula empirica (notazione di Hill):
C17H11N3O2
Numero CAS:
27030-97-3
Peso molecolare:
289.29
Beilstein:
755407
Numero CE:
248-173-3
Numero MDL:
MFCD00042767
Codice UNSPSC:
12352200
ID PubChem:
57646978

Nome Commerciale

BioReagent

Saggio

≥98.0% (HPLC)

Stato

solid

Solubilità

H2O: soluble
acetone: soluble

Fluorescenza

λex 315 nm; λem 365 nm (after derivatization with 2-mercaptoethanol)

Compatibilità

suitable for fluorescence

Stringa SMILE

O=C1C=CC(=O)N1c2ccc(cc2)-c3nc4ccccc4[nH]3

InChI

1S/C17H11N3O2/c21-15-9-10-16(22)20(15)12-7-5-11(6-8-12)17-18-13-3-1-2-4-14(13)19-17/h1-10H,(H,18,19)
NZDOXVCRXDAVII-UHFFFAOYSA-N

Altre note

Fluorescent probe in proteins, used for microfluorometry of thiol compounds (detection limit: 10-6 m thiol conc.); Review

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number

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K Taniguchi et al.
The Journal of biological chemistry, 258(11), 6927-6931 (1983-06-10)
The addition of ATP to (Na+,K+)-ATPase (EC 3.6.1.3) of pig kidney modified with N-[p-(2-benzimidazolyl)phenyl]maleimide induced three different modes of fluorescence change: positive, biphasic, and negative, which were observed, respectively, in the presence of Mg2+ with 16 or 160, 320 or
Robert J W Brewin et al.
Sensors (Basel, Switzerland), 19(4) (2019-03-01)
Two expanding areas of science and technology are citizen science and three-dimensional (3D) printing. Citizen science has a proven capability to generate reliable data and contribute to unexpected scientific discovery. It can put science into the hands of the citizens
K Taniguchi et al.
Journal of biochemistry, 109(2), 299-306 (1991-02-01)
Oligomycin reduced the fluorescence intensity of an N-(p-(2-benzimidazoly)phenyl) maleimide (BIPM) probe at Cys-964 of the alpha-chain of pig kidney Na+,K(+)-ATPase with increase in the concentration of Na+ with a Hill coefficient of nh = 0.77 with Kh = 231 mM.
S Inoue et al.
Japanese journal of pharmacology, 43(1), 107-111 (1987-01-01)
Trypsin treatment of N-[p-(2-benzimidazolyl)phenyl]maleimide modified enzyme caused a marked reduction in Na+,K+-ATPase activity and in the amount of the alpha-chain, which contains the phosphorylation and ouabain binding sites. However, these preparations retained nearly 90% of the ouabain binding capacity and
Fluorescent thiol reagents. V. Microfluorometry of thiol compounds with a fluorescent-labeled maleimide.
T Sekine et al.
Analytical biochemistry, 48(2), 557-568 (1972-08-01)
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