11758241001
Roche
β-Gal Reporter Gene Assay, chemiluminescent
sufficient for 500 assays (microplate), sufficient for 250 assays (tubes)
Sinonimo/i:
galactosidase
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About This Item
Codice UNSPSC:
23201100
Prodotti consigliati
impiego
sufficient for 250 assays (tubes)
sufficient for 500 assays (microplate)
Produttore/marchio commerciale
Roche
Temperatura di conservazione
2-8°C
Descrizione generale
The β-Gal Reporter Gene Assay is designed for specifically measuring bacterial β-galactosidase activity. To achieve this, the enzyme reaction is conducted at a pH that is optimized for bacterial β-Gal, but allows no significant endogenous eukaryotic β-galactosidase activity. However, if very high endogenous β-galactosidase activity is found (e.g., with hepatic cells), heat treatment can be performed to ensure the specific determination of the amount of bacterial β-galactosidase that is encoded by the transfected plasmid.
Applicazioni
The β-Gal Reporter Gene Assay, chemiluminescent, is used to quantitatively measure β-Gal expression in eukaryotic cells that are transfected with a plasmid bearing the β-Gal-encoding lacZ reporter gene.
Caratteristiche e vantaggi
- Sensitive: Chemiluminescence technology allows detection of approximately 20 fg β-galactosidase in cell extracts
- High dynamic measuring range: Linear range over four to five orders of magnitude
- Constant light emission: The assay produces a long-lasting light emission instead of a short-peak kinetic
- Safe: No radioactive isotopes are used
- Fast: Approximately 1.5 to 2.5 hours elapse from start to finish
- Convenient: Kit contains all reagents needed, including lysis buffer, protease inhibitors, and a positive control
Confezionamento
1 kit containing 7 components.
Nota sulla preparazione
Storage conditions (working solution): Substrate Reagent (solution 1) is stable at least for 24 h at 2 to 8 °C.
Initation Reagent (solution 2) is stable for at least 4 weeks when stored at 2 to 8 °C.
Lysis Reagent, 1x (solution 3) Lysis reagent without protease inhibitors is stable at
-15 to -25 °C or for 3 months at 2 to 8 °C. Lysis reagent containing protease inhibitors is stable for one week when stored frozen at -15 to -25 °C.
Positive Control (solution 4) The reconstituted enzyme solution is stable for 1 week at 2 to 8 °C. When stored frozen in aliquots at -15 to -25 °C the enzyme is stable for 3 months. Avoid multiple freezing and thawing.
Initation Reagent (solution 2) is stable for at least 4 weeks when stored at 2 to 8 °C.
Lysis Reagent, 1x (solution 3) Lysis reagent without protease inhibitors is stable at
-15 to -25 °C or for 3 months at 2 to 8 °C. Lysis reagent containing protease inhibitors is stable for one week when stored frozen at -15 to -25 °C.
Positive Control (solution 4) The reconstituted enzyme solution is stable for 1 week at 2 to 8 °C. When stored frozen in aliquots at -15 to -25 °C the enzyme is stable for 3 months. Avoid multiple freezing and thawing.
Altre note
For life science research only. Not for use in diagnostic procedures.
Solo come componenti del kit
N° Catalogo
Descrizione
- β-Gal Substrate, Galacton Plus 100x concentrated
- Assay Buffer
- Enhancer
- Initiation Solution
- Protease Inhibitors
- Lysis Buffer 5x concentrated
- Positive Control, β-galactosidase (E. coli), lyophilizate
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Classi di pericolo
Aquatic Chronic 3 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
Organi bersaglio
Central nervous system
Codice della classe di stoccaggio
3 - Flammable liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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