10108626001
Roche
Poly(A)
lyophilized, suitable for PCR, pkg of 100 mg
Sinonimo/i:
Poly(A), Polyadenylic acid
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About This Item
Codice UNSPSC:
41105600
Prodotti consigliati
Descrizione
Polyadenylic acid
Livello qualitativo
Forma fisica
lyophilized
PM
700-3500 kDa
Confezionamento
pkg of 100 mg
Produttore/marchio commerciale
Roche
Concentrazione
0.5 mg/mL (Working concentration)
tecniche
PCR: suitable
Colore
white
Solubilità
water: soluble
Rapporto di assorbanza
A290/260 nm 0.03-0.05
A280/260 nm 0.28-0.32
A250/260 nm 0.86-0.90
Temperatura di conservazione
2-8°C
Descrizione generale
Poly(A) is used as a carrier for quantitative precipitation of DNA and RNA.
Applicazioni
Poly(A) has been used for droplet digital PCR (ddPCR) assay.
Polyadenylic acid (Poly(A)) to inhibit Exo1′s exonuclease activity. It has also been used as a carrier to resuspend lyophilized gBlocks.
Azioni biochim/fisiol
Polyadenylic acid (Poly(A)) tails present at 3′ end are produced in the cell nucleus. It contains ~250 nucleotides in mammalian cells. Poly(A) regulates mRNA decay and the initiation of translation. Cytoplasmic poly(A) extension modulates translation.
Qualità
Typical analysis:
2.3μmol/mg Poly(A) (from absorbance) in relation to one mononucleotide unit. Chromatographically homogeneous.
Absorbance determination A250/A260, A280/A260, A290/A260
2.3μmol/mg Poly(A) (from absorbance) in relation to one mononucleotide unit. Chromatographically homogeneous.
Absorbance determination A250/A260, A280/A260, A290/A260
Sequenza
Chain Length 2.100 to 10.000 nucleotides
Definizione di unità
1 A260 unit corresponds to 40 μg ssRNA.
Stato fisico
Lyophilizate, potassium salt
Nota sulla preparazione
Working concentration: 0.5 mg/ml
A concentration of 0.5 mg/ml is recommended.
Working solution: 0.5 mg/ml is recommended.
A concentration of 0.5 mg/ml is recommended.
Working solution: 0.5 mg/ml is recommended.
Altre note
For life science research only. Not for use in diagnostic procedures.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Poly (A) tail length is controlled by the nuclear poly (A)-binding protein regulating the interaction between poly (A) polymerase and the cleavage and polyadenylation specificity factor
Kuhn U, et al.
The Journal of Biological Chemistry, 284(34), 22803-22814 (2009)
Yuichiro Miyaoka et al.
Scientific reports, 6, 23549-23549 (2016-04-01)
Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-directed repair (HDR). However, nonhomologous end-joining (NHEJ), an error-prone repair, acts concurrently, reducing the rate of high-fidelity edits. The identification of genome-editing conditions that
Poly (ADP-ribose)-binding promotes Exo1 damage recruitment and suppresses its nuclease activities
Cheruiyot A, et al.
DNA Repair, 35, 106-115 (2015)
Kengo Watanabe et al.
Nature communications, 12(1), 1353-1353 (2021-03-03)
Cells are under threat of osmotic perturbation; cell volume maintenance is critical in cerebral edema, inflammation and aging, in which prominent changes in intracellular or extracellular osmolality emerge. After osmotic stress-enforced cell swelling or shrinkage, the cells regulate intracellular osmolality
Peiguo Yang et al.
Cell, 181(2), 325-345 (2020-04-18)
The mechanisms underlying ribonucleoprotein (RNP) granule assembly, including the basis for establishing and maintaining RNP granules with distinct composition, are unknown. One prominent type of RNP granule is the stress granule (SG), a dynamic and reversible cytoplasmic assembly formed in
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