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SCR030

Sigma-Aldrich

Human Embryonic Germ Layer Characterization Kit

CHEMICON′s Human Embryonic Germ Layer Characterization Kit allows researchers to assess the differentiation potential of their Human Embryonic Stem Cell (HESC) to form derivatives of all three embryonic germ layers.

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About This Item

Codice UNSPSC:
12352207
eCl@ss:
32161000
NACRES:
NA.84

Livello qualitativo

Reattività contro le specie

human

Produttore/marchio commerciale

Chemicon®

tecniche

cell culture | stem cell: suitable
immunocytochemistry: suitable

input

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

Condizioni di spedizione

wet ice

Descrizione generale

Human embryonic stem cells (HESC) are pluripotent stem cells that are capable of self-renewal and of differentiation in vitro and in vivo into a wide variety of cell types, including derivatives of all three embryonic germ layers. These primary germ layers will eventually give rise to all the tissues and organs of the body.



Mesoderm: bone, cartilage, muscle, blood and vascular system

Endoderm: gastrointestinal tract, respiratory tract and endocrine glands (including liver and pancreas)

Ectoderm: nervous system, skin



CHEMICON®′s Human Embryonic Germ Layer Characterization Kit (SCR030) allows researchers to assess the differentiation potential of their HESC to form derivatives of all three embryonic germ layers. Included in the kit are antibodies that are routinely used by stem cell researchers to identify the lineage fates of HESC following either spontaneous or directed differentiation.



Mesoderm: Smooth Muscle Actin (CBL171)

Cardiac Troponin I (MAB1691-50UG)



Endoderm: Alpha Fetoprotein (2004189)



Ectoderm: Nestin (ABD69)

MAP-2 (MAB3418-50UG)

Applicazioni

CHEMICON′s Human Embryonic Germ Layer Characterization Kit allows researchers to assess the differentiation potential of their Human Embryonic Stem Cell (HESC) to form derivatives of all three embryonic germ layers.
Research Category
Stem Cell Research

Componenti

50 μL of Alpha Fetoprotein mouse monoclonal 2004189

50 μg of Smooth Muscle Actin mouse monoclonal CBL171

50 μL of Nestin rabbit polyclonal ABD69

50 μg of Cardiac Troponin I mouse monoclonal MAB1691-50UG

50 μg of MAP-2 mouse monoclonal MAB3418-50UG

Stoccaggio e stabilità

Maintain components according to the individual data sheets. Antibodies to alpha fetoprotein (2004189) and nestin (ABD69) are to be stored at -20°C (SCR030 - Part 1). Antibodies to smooth muscle actin (CBL171), cardiac troponin I (MAB1691-50UG) and MAP-2 (MAB3418-50UG) are to be stored at 2 - 8°C (SCR030 - Part 2).

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pittogrammi

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Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3

Codice della classe di stoccaggio

10 - Combustible liquids


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Journal of biomolecular screening, 15(6), 630-643 (2010-06-10)
Techniques to evaluate gene expression profiling, including real-time quantitative PCR, TaqMan low-density arrays, and sufficiently sensitive cDNA microarrays, are efficient methods for monitoring human embryonic stem cell (hESC) cultures. However, most of these high-throughput tests have a limited use due

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