MABS2000

Anti-LXR alpha Antibody, clone 2B7

clone 2B7, from mouse

• Sinonimo/i: Oxysterols receptor LXR-alpha, Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3, RLD-1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 2B7, monoclonal
• Reattività contro le specie: mouse, rat
• Confezionamento: antibody small pack of 25 μg
• tecniche: immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso UniProt: Q62685
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Nr1H3(22259)

Descrizione

Descrizione generale

Oxysterols receptor LXR-alpha (UniProt: Q62685; also known as Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3, RLD-1) is encoded by the Nr1h3 (also known as Lxra) gene in rat. Liver X receptors (LXRs) are members of the nuclear receptor superfamily that have been implicated in lipid homeostasis and glucose metabolism. Oxysterols, oxidized derivatives of cholesterol, are ligands for LXRs. LXRs act as cholesterol sensors; when cellular oxysterols accumulate as a result of increasing concentrations of cholesterol, LXR induces the transcription of genes that protect cells from cholesterol overload. LXRs bind to consensus elements (LXR response elements, LXREs) as heterodimers with isoforms of the retinoid X receptor (RXR). LXR-alpha is an important regulator of cholesterol and bile acid metabolism. It interacts with RXR and shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. In adults it is expressed in spleen, pituitary, lung, liver, and fat and a weaker expression is observed in several other tissues. LXR-alpha contains a transactivation domain (AF-1; aa 1-94) that is required for ligand-independent transactivation function; another transactivation domain (AF-2; aa 203-445) that is required for ligand-dependent transactivation function; and a ligand binding domain (aa 213-432). (Ref.: Zhao, C., and Dahlman-Wright, K. (2010). J. Endocrinol. 204, 233-240).

Specificità

Clone 2B7 detects LXR-alpha in mouse and rat liver tissue.

Immunogeno

GST-tagged full length recombinant rat LXR-alpha protein.

Applicazioni

Anti-LXR alpha, clone 2B7, Cat. No. MABS2000, is a mouse monocloanl antibody that detects Oxysterols receptor LXR-alpha and has been tested for use in Immunoprecipitation and Western Blotting.

Immunoprecipitation Analysis: A representative lot detected LXR alpha in Immunoprecipitation applications (Tian, J., et. al. (2016). Proc Natl Acad Sci USA. 113(29):8182-7).

Western Blotting Analysis: A representative lot detected LXR alpha in Western Blotting applications (Tian, J., et. al. (2016). Proc Natl Acad Sci USA. 113(29):8182-7).

Research Category
Signaling

Qualità

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: 1 ug/mL of this antibody detected LXR alpha in 10 µg of mouse liver tissue lysate.

Descrizione del bersaglio

~50 kDa observed; 50.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable