MABS1300

Anti-asparagine synthetase Antibody, clone Z5808

clone Z5808, from mouse

• Sinonimo/i: Asparagine synthetase [glutamine-hydrolyzing], Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: Z5808, monoclonal
• Reattività contro le specie: human
• tecniche: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_001664
• N° accesso UniProt: P08243
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ASNS(440)

Descrizione

Descrizione generale

Asparagine synthetase [glutamine-hydrolyzing] (UniProt: P08243; also known as EC:6.3.5.4, Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase) is encoded by the ASNS (also known as TS11) gene (Gene ID: 440) in human. Asparagine synthetase catalyzes the biosynthesis of L-asparagine from L-aspartate in an ATP-dependent manner. It contains one asparagine synthetase domain and one glutamine amidotransferase type-2 domain. ASNS transcription is enhanced by amino acid deprivation via a signal transduction pathway that senses amino acid availability. Mutations in ASNS gene are reported to result in asparagine synthetase deficiency that is characterized by microcephaly, severely delayed psychomotor development, progressive encephalopathy, and hyperekplexic activity. There has been much interest in the development of asparagine synthetase inhibitors for treatment of asparaginase-resistance leukemias and levels of asparagine synthetase have been considered as a potential biomarker in ovarian cancers.

Specificità

Clone Z5808 detects Asparagine synthetase in human creast cancer, human tonsil, and human uterus. It targets an epitope within 50 amino acids from the N-terminal end.

Immunogeno

N-asparagine synthetase-budded virus.

Applicazioni

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected asparagine synthetase in human tonsil and human uterus tissues.

Immunocytochemistry Analysis: A representative lot detected asparagine synthetase in Immunocytochemistry applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32).

Flow Cytometry Analysis: A representative lot detected asparagine synthetase in Flow Cytometry applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32).

ELISA Analysis: A representative lot detected asparagine synthetase in ELISA applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32).

Western Blotting Analysis: A representative lot detected asparagine synthetase in Western Blotting applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32).

This mouse monoclonal Anti-asparagine synthetase, clone Z5808, Cat. No. MABS1300 has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of human Asparagine Synthetase.

Qualità

Evaluated by Immunohistochemistry in human breast cancer tissues.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected asparagine synthetase in human breast cancer tissue.

Descrizione del bersaglio

64.37 kDa calculated.

Stato fisico

Format: Purified

Purified mouse monoclonal antibody IgG2a in PBS with 0.01% sodium azide.

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable