MABN2435

Anti-Peripherin-2 Antibody, clone 5H2

clone 5H2, from mouse

• Sinonimo/i: Retinal degeneration slow protein
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 5H2, monoclonal
• Reattività contro le specie: bovine, mouse
• Confezionamento: antibody small pack of 25 μg
• tecniche: immunofluorescence: suitable; radioimmunoassay: suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso UniProt: P17810
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Prph2(19133)

Descrizione

Descrizione generale

Peripherin-2 (UniProt: P17810; also known as Retinal degeneration slow protein) is encoded by the PRPH2 (also known as RDS) gene in bovine species. Peripherin-2 is a disulfide-linked homodimeric, multi-pass membrane protein that may function as an adhesion molecule involved in stabilization and compaction of outer segment disks in retina and is considered to be essential for disk morphogenesis. It is exclusively expressed in outer segments. Peripherin-2 can form homo- and heteromeric protein complexes in outer segment. The core homomeric peripherin-2 unit is the non-covalent tetramer, which can also assemble into covalently linked octamers and higher-order oligomers. The homomeric peripherin-2 complexes are essential for proper outer segment morphology and architecture. Peripherin-2 is also shown to form heteromeric complexes with its homolog ROM-1. Mutations in PRPH2 gene can lead to autosomal dominant retinitis pigmentosa (adRP) And a majority of these mutations are located within the large loop domain connecting the transmembrane domain 3 and 4 (Ref.: Bohm, S et al. (2017). Scientific Reports 7, Article number: 2321).

Specificità

Clone 5H2 detects Peripherin-2 in bovine and mouse retina.

Immunogeno

Epitope: C-terminus

Purified bovine whole peripherin.

Applicazioni

Anti-Peripherin-2, clone 5H2, Cat. No. MABN2435, is a highly specific mouse monoclonal antibody that targets Peripherin-2 and has been tested in Immunofluorescence, Radioimmunoassay, and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A representative lot detected Peripherin-2 in Western Blotting applications (Connell, G., et. al. (1991). Proc Natl Acad Sci USA. 88(3):723-6; Zhang, Y., et. al. (2009). J Cell Sci. 122(Pt 8):1192-200).

Radioimmunoassay Analysis: A representative lot detected Peripherin-2 in Radioimmunoassay applications (Connell, G., et. al. (1991). Proc Natl Acad Sci USA. 88(3):723-6).

Immunofluorescence Analysis: A representative lot detected Peripherin-2 in Immunoflourescence applications (Zhang, Y., et. al. (2009). J Cell Sci. 122(Pt 8):1192-200).

Qualità

Evaluated by Western Blotting in bovine retinal tissue lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected Peripherin-2 in 10 µg of bovine retinal tissue lysate.

Descrizione del bersaglio

~39 kDa observed; 38.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash