MABF249

Anti-Tapasin Antibody, clone 7F6

clone 7F6, from rat

• Sinonimo/i: Tapasin, TPN, TSPN, NGS-17, TAP-associated protein, TAP-binding protein
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rat
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 7F6, monoclonal
• Reattività contro le specie: human
• tecniche: flow cytometry: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: np_003181
• N° accesso UniProt: O15533
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TAPBP(6892)

Descrizione

Descrizione generale

Tapasin (TPN or TPSN) is also known as NGS-17, TAP-associated protein and TAP-binding protein. Tapasin plays a role in MHC class I peptide loading and assembly, and is involved in associating MHC class I with transporter associated with antigen processing (TAP). Tapasin is found in neutrophils, predominantly within fully differentiated cells.

Specificità

This antibody recognizes the Lumenal domain of Tapasin.

Immunogeno

Linear peptide corresponding to human Tapasin.

Applicazioni

Detect Tapasin using this rat monoclonal antibody, Anti-Tapasin Antibody, clone 7F6 validated for use in western blotting & Flow Cytometry.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Tapasin in 10 µg of A431, HEK293, HepG2, HUVEC, Jurkat, and PC12 cell lysates.

Flow Cytometry Analysis: 4 µg from a representative lot detected Tapasin in 1X10E6 PBMC cells.

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA transfected MJS cell lysate (Hislop, A. D., et al. (2007). J Exp Med. 204(8):1863-1873.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in HeLa cell lysate (Hulpke, S., et al. (2012). Cell Mol Life Sci. 69(19):3317-3327.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA4M transfected MJS cell lines (Horst, D., et al. (2009). J Immunol. 182(4):2313-2314.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in WB (Koch, J., et al. (2006). FEBS Lett. 580(17):4091-4096.).

Qualità

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Tapasin in 10 µg of HeLa cell lysate.

Descrizione del bersaglio

~48 kDa observed

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable