MABE923

Anti-Histone H3, clone 1B1-B2 Antibody

clone 1B1-B2, from mouse

• Sinonimo/i: Histone H3.3C, Histone H3.5
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 1B1-B2, monoclonal
• Reattività contro le specie: yeast, mouse, human
• Reattività contro le specie (prevista in base all’omologia): vertebrates (based on 100% sequence homology)
• tecniche: ChIP: suitable; western blot: suitable
• Isotipo: IgG3κ
• N° accesso NCBI: NP_001013721
• N° accesso UniProt: Q6NXT2
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... H3C1(8350) ; mouse ... H3C1(360198)

Descrizione

Descrizione generale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation.

Immunogeno

Epitope: C-terminus

Linear peptide corresponding to the C-terminus of human Histone H3.

Applicazioni

Anti-Histone H3 antibody, clone 1B1-B2 is an antibody against Histone H3 for use in western blotting & ChIP.

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H3 from mouse embyonic fibroblasts (MEF).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Qualità

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Histone H3 in 10 µg of HeLa cell lysate.

Descrizione del bersaglio

~15 kDa observed

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable