MAB1810-I

Anti-ADAMTS-1 Antibody

purified antibody, clone 5D4E11B6, from mouse

• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 5D4E11B6, monoclonal
• PM: calculated mol wt 105.36 kDa; observed mol wt ~58 kDa
• Reattività contro le specie: mouse, human
• Reattività contro le specie (prevista in base all’omologia): rat
• Confezionamento: antibody small pack of 100 μg
• tecniche: ELISA: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• Isotipo: IgG2κ
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Specificità

Clone 5D4E11B6 is a mouse monoclonal antibody that detects A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS-1).

Immunogeno

Myc-His-tagged full length recombinant human ADAMTS-1.

Applicazioni

Quality Control TestingEvaluated by Western Blotting in HEK293 cell lysate.Western Blotting Analysis: A 1:125 dilution of this antibody detected ADAMTS-1 in HEK293 cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:125 dilution from a representative lot detected ADAMTS-1 in lysates from A431 cells and E16 mouse embryonic brain tissue.Western Blotting Analysis: A representative lot detected ADAMTS-1 in Western Blotting applications (Meng, T.G., et. al. (2017). Cell Death Dis. 8(6):e2846; Luque, A., et. al. (2003). J Biol. Chem. 278 (26); 23656-65).Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected ADAMTS-1 in HeLa cells.ELISA Analysis: A representative lot detected ADAMTS-1 in ELISA applications (Luque, A., et. al. (2003). J Biol Chem. 278(26); 23656-65).Immunofluorescence Analysis: A representative lot detected ADAMTS-1 in Immunofluorescence applications (Silva, S.V., et. al. (2016). PLoS One. 11(10):e0165061; Luque, A., et. al. (2003). J Biol Chem. 278(26); 23656-65).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-ADAMTS-1, clone 5D4E11B6, Cat. No. MAB1810-I, is a mouse monoclonal antibody that detects ADAMTS-1 and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Western Blotting.

Descrizione del bersaglio

A disintegrin and metalloproteinase with thrombospondin motifs 1 (UniProt: Q9UHI8; also known as ADAM-TS 1, ADAM-TS1, ADAMTS-1, METH-1) is encoded by the ADAMTS1 (also known as KIAA1346, METH1) gene (Gene ID: 9510) in human. ADAM-TS1 is a zinc-binding protein that is synthesized with a signal peptide (aa 1-49) and a propeptide (aa 50-252) that are subsequently cleaved by furin endopeptidase to generate the mature form (aa 253-967). It has a conserved cysteine in the cysteine-switch motif that binds the catalytic zinc ion and inhibits the enzyme. The dissociation of cysteine from the zinc ion upon the activation-peptide release activates the enzyme. It also contains spacer domain (aa 725-849) and the TSO-1 domain that are important for a tight interaction with the extracellular matrix. ADAM-TS1 cleaves aggrecan, a cartilage proteoglycan at Glu1938-Leu1939 site within the chondroitin sulfate attachment domain and is involved in its turnover. ADAMTS1 is implicated in vascularization and development and displays anti- and pro-tumorigenic activities. ADAMTS1 knockout mice exhibit reduced body weight, kidney malformation, and impaired female fertility. (Ref.: Rodriguez-Baena, FJ., et al. (2018). Sci. Rep. 8: Article 13103).

Stato fisico

Purified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at +2°C to +8°C from date of receipt.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable