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Sigma-Aldrich

Anti-Cathepsin D (Ab-2) Rabbit pAb

liquid, Calbiochem®

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About This Item

Codice UNSPSC:
12352203

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

liquid

contiene

≤0.1% sodium azide as preservative

Reattività contro le specie

human

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

do not freeze

Isotipo

IgG

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... CTSD(1509)

Descrizione generale

Anti-Cathepsin D (Ab-2), rabbit polyclonal, recognizes the ~52 kDa latent form and ~34 kDa subunit in breast carcinoma tissue. It is validated for WB and IHC with frozen and paraffin sections.
Purified rabbit polyclonal antibody. Recognizes the ~52 kDa latent form and the ~34 kDa (but not the ~14 kDa) active subunit of cathepsin D.
Recognizes the ~52 kDa latent form D and the ~34 kDa subunit, but not the ~14 kDa subunit of cathepsin D in breast carcinoma tissue extracts.

Immunogeno

Human
purified human liver cathepsin D

Applicazioni

Frozen Sections (5 µg/ml)

Immunoblotting (2.5 µg/ml)

Paraffin Sections (5 µg/ml, trypsin pre-treatment required)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Risultati analitici

Positive Control
HT1080 concentrated conditioned medium or breast carcinoma tissue

Altre note

For optimal results in immunoblotting, conditioned media from HT1080 cells should be concentrated 10-20X prior to loading. Antibody should be titrated for optimal results in individual systems.
Rochefort, H. 1992. Eur. J. Cancer28A, 1780.
Rochefort, H. 1990. In Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980. Cell20, 352.
Barret, A. J. 1977. In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

10-13 - German Storage Class 10 to 13


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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