FCMAB101A4

Milli-Mark^® Anti-BrdU Antibody, clone BU-1 Alexa Fluor^™ 488 conjugated

clone Bu-1, Milli-Mark^®, from mouse

• Sinonimo/i: BrdU
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Coniugato: ALEXA FLUOR^™ 488
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: Bu-1, monoclonal
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): all
• Produttore/marchio commerciale: Milli-Mark^®
• tecniche: flow cytometry: suitable; immunocytochemistry: suitable
• Isotipo: IgG2a
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. This antibody does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU. The antibody′s special ability is thought to be due to the presence of DNAse in the antibody solution arising from the mycoplasma infecting the original hybridoma line {Gonchoroff, 1985}.

Specificità

Specifically binds to bromodeoxyuridine (crossreacts with iodouridine 10%). Does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine.

Immunogeno

Bromodeoxyuridine

Applicazioni

Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor 488 conjugated detects level of BrdU & has been published & validated for use in FC & IC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Qualità

Evaluated by flow cytometry with Jurkat Cells.

Descrizione del bersaglio

none given

Stato fisico

Presentation needs to say: Purified mouse monoclonal IgG2a supernatant conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. Possible mycoplasma risk contained within this product. Use appropriate measures to prevent cell line infection/transmission.

Protein G Purified

Stoccaggio e stabilità

Stable for 6 months at 2-8°C from date of receipt. Protect from light.

Risultati analitici

Control
After incorporation of BrdU, all DNA containing species

Note legali

ALEXA FLUOR is a trademark of Life Technologies

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable