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DR1002

Sigma-Aldrich

PhosphoDetect Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12)

liquid, clone 10H11.E12, Calbiochem®

Sinonimo/i:

Anti-Ataxia Telangiectasia, Phospho-Specific (Ser¹⁹⁸¹)

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About This Item

Codice UNSPSC:
12352203

Origine biologica

mouse

Livello qualitativo

Tipo di anticorpo

primary antibodies

Clone

10H11.E12, monoclonal

Stato

liquid

contiene

≤0.1% sodium azide as preservative

Reattività contro le specie

human, mouse

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

OK to freeze
avoid repeated freeze/thaw cycles

Isotipo

IgG1

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Descrizione generale

Protein G purified mouse monoclonal antibody. Recognizes the ~370 kDa ATM protein phosphorylated at Ser1981.
Recognizes the ~370 kDa ATM protein phosphorylated on Ser1981 in camptothecin-treated HeLa cells. Sold under license of U.S. Patent 7,108,992.
This PhosphoDetect Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) is validated for use in Immunoblotting, Immunocytochemistry for the detection of ATM (pSer¹⁹⁸¹).

Immunogeno

Human
a synthetic phosphopeptide (SLAFEEGpSQSTTISS) corresponding to amino acids 1974-1988 surrounding the Ser¹⁹⁸¹ phosphorylation site of human ATM

Applicazioni





Immunoblotting (0.5 g/ml)
Immunocytochemistry (1.5 g/ml)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Irritant (B)

Stato fisico

In PBS.

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C).

Risultati analitici

Positive Control
HeLa cells treated with camptothecin (Cat. No. 208925)

Altre note

Does not detect the unphosphorylated protein. Use 5% milk in TBS-T for blocking; the use of BSA is not recommended. The use of PBS-T is not recommended, as phosphate-containing buffers can contribute to high background with phosphospecific antibodies. Antibody should be titrated for optimal results in individual systems.
Horejsi, Z., et al. 2004. Oncogene23, 3122.
Abraham, R.T. 2003. Genes Dev.15, 2177.
Bakkenist, C.J. and Kastan, M.B. 2003. Nature421, 499.
Shiloh, Y. 2003. Nat. Rev. Cancer3, 155.

Note legali

Sold under license of U.S. Patent 7,108,992.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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