ABS492

Anti-RhoGAP1 Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Rho GTPase-activating protein 1, CDC42 GTPase-activating protein, GTPase-activating protein rhoOGAP, Rho-related small GTPase protein activator, Rho-type GTPase-activating protein 1, p50-RhoGAP, RhoGAP1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: mouse, rat, human
• tecniche: immunocytochemistry: suitable; western blot: suitable
• N° accesso NCBI: NP_004299
• N° accesso UniProt: Q07960
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ARHGAP1(392)

Descrizione

Descrizione generale

RhoGAP1, also known as Rho GTPase-activating protein 1, CDC42 GTPase-activating protein, Rho-related small GTPase protein activator, Rho-type GTPase-activating protein 1, or p50-RhoGAP, and encoded by the gene ARHGAP1/CDC42GAP/RHOGAP1, is an important GTPase activator for the Rho, Rac, and CDC42 GTP binding proteins, converting them to the putatively inactive GDP-bound state. CDC42 is the preferred substrate for RhoGAP1. Such GTPase-activating proteins or GAPs play an important role in signal transduction pathways regulated by GTP-binding proteins. In addition to acting as down-regulators of GTPases, there is growing evidence that they also act as effector molecules required for downstream signaling. RhoGAP1 is found in multiprotein complexes in the cytoplasm in most cells. Recent research has shown RhoGAP1 to be particularly important during epithelial to mesenchymal morphogenesis and for carcinoma metastasis. Rho-ROCK signaling is essential for cell apical detachment and the generation of motility. RhoGAP1 restricts Rho activation to apical areas and thus helps guide the detachment process and thus is a target of much developmental and cancer research.

Immunogeno

GST-tagged recombinant protein corresponding to human RhoGAP1.

Applicazioni

Anti-RhoGAP1 Antibody is an antibody against RhoGAP1 for use in western blotting & ICC.

Research Category
Signaling

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected RhoGAP1 in 10 µg of A431, NIH/3T3, and PC-12 cell lysates.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected RhoGAP1 in HeLa cells (Schiel, J. A., et al. (2012). Nat Cell Biol. 14(10):1068-1078.).

Qualità

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected RhoGAP1 in 10 µg of HeLa cell lysate.

Descrizione del bersaglio

~50 kDa observed

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable