ABE416

Anti-SHMT1 Antibody

from sheep, purified by affinity chromatography

• Sinonimo/i: Serine hydroxymethyltransferase, cytosolic, SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: sheep
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human, mouse
• tecniche: ChIP: suitable; western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NP_033197
• N° accesso UniProt: P50431
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SHMT1(6470)

Descrizione

Descrizione generale

Serine hydroxymethyltransferase (SHMT) is also known as glycine hydroxymethyltransferase or serine methylase, and is an enzyme that catalyzes the reversible conversion of serine and glycine. SHMT is thought to be involved in the epigenetic DNA methylation mechanism and has shown increased activity during cell proliferation and the S phase of the cell cycle. Evidence indicates that SHMT repression is a critical step in the Retinoic Acid-induced cell growth arrest and differentiation pathway.

Immunogeno

KLH-conjugated linear peptide corresponding to mouse SHMT1.

Applicazioni

Anti-SHMT1 Antibody is a highly specific sheep polyclonal antibody, that targets Serine hydroxymethyltransferase & has been tested in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Western Blot Analysis: 2.5 µg/mL of this antibody detected SHMT1 in 10 µg of mouse liver tissue lysate (20 µL of antibody at 5 mg/mL is provided. Please take caution to centrifuge and handle carefully, when pipetting from the tube.).

Chromatin Immunoprecipitation: A representative lot from an independent laboratory detected SHMT1 when coprecipitated with PCNA in pBABE-Puro-SV50-LT transfected HeLa nuclear extracts. MacFarlane A.J., et al. (2011). J Biol Chem. 286(51):44015-22

Qualità

Evaluated by Western Blot in human liver tissue lysate.

Western Blot Analysis: 2.5 µg/mL of this antibody detected SHMT1 in 10 µg of human liver tissue lysate (20 µL of antibody at 5 mg/mL is provided. Please take caution to centrifuge and handle carefully, when pipetting from the tube.).

Descrizione del bersaglio

~53 kDa observed. An uncharacterized band may be observed at ~40 kDa in some tissue lysates.

Stato fisico

Affinity purified

Purified sheep polyclonal in buffer containing 0.5M HEPES with 0.05% sodium azide and 50% glycerol.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Risultati analitici

Control
Human liver tissue lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1