ABE2696

Anti-GADD45 alpha Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Growth arrest and DNA damage-inducible protein GADD45 alpha, DDIT-1, DNA damage-inducible transcript 1 protein
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: mouse, human
• Reattività contro le specie (prevista in base all’omologia): porcine (based on 100% sequence homology), bovine (based on 100% sequence homology)
• tecniche: immunohistochemistry: suitable; western blot: suitable
• N° accesso NCBI: NP_001915
• N° accesso UniProt: P24522
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... GADD45A(1647)

Descrizione

Descrizione generale

Growth arrest and DNA damage-inducible protein GADD45 alpha (UniProt P24522; also known as DDIT-1, DNA damage-inducible transcript 1 protein) is encoded by the GADD45A (also known as DDIT1, GADD45) gene (Gene ID 1647) in human. GADD45A, GADD45B, and GADD45G constitute a family of sensors that can mediate rapid responses to a variety of stressors in mammals. GADD45 proteins are involved in cellular DNA repair, cell cycle, cell survival, apoptosis, senescence, and DNA demethylation under various stress conditions. The interaction of GADD45 with PCNA regulates DNA replication and repair by stimulating nucleotide excision repair. GADD45 can induce cell cycle arrest through direct interaction with cell cycle-related protein CDK1 and p21. Direct phosphorylation of MEKK4 by GADD45 leads to activation of JNK and p38 kinase, resulting in cellular apoptosis and senescence, respectively. All GADD45 proteins are able to interact with each other and form homo- or hetero-dimers, which are crucial for GADD45 functions. GADD45 proteins are ubiquitously expressed, but can vary in their tissue expression patterns among the three members. Gadd45a deletion in mice causes genomic instability, resulting in defective mitosis and cytokinesis, as wll as increased radiation carcinogenesis. In addition to the reported antitumor activity via the induction of cellular apoptosis or senescence in cancer cells, GADD45A has also been shown to play a role in facilitating tumorigenesis in certain cancers.

Specificità

This polyclonal antibody detects GADD45 alpha. Immunogen sequence is not conserved in GADD45 beta.

Immunogeno

Epitope: N-terminus.

KLH-conjugated linear peptide corresponding to the N-terminal sequence of human GADD45 alpha.

Applicazioni

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

This Anti-GADD45 alpha Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of GADD45 alpha.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected GADD45 alpha dimer in 10 µg of HEK293 cell lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GADD45 alpha in human colon cancer tissue section.

Qualità

Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected GADD45 alpha in 10 µg of mouse kidney tissue lysate.

Descrizione del bersaglio

~18/36 kDa (monomer/dimer) observed. 18.34 kDa (monomer) calculated. Uncharacterized band(s) may appear in some lysates.

Linkage

Replaces: 07-1230

Stato fisico

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable