ABE1836

Anti-SMARCAL1 Antibody

serum, from rabbit

• Sinonimo/i: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, HepA-related protein, hHARP, Sucrose nonfermenting protein 2-like 1, SMARCAL1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: serum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: human, mouse
• tecniche: immunoprecipitation (IP): suitable; western blot: suitable
• N° accesso NCBI: NP_001120679
• N° accesso UniProt: Q9NZC9
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SMARCAL1(50485)

Descrizione

Descrizione generale

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 (UniProt Q9NZC9; also known as ATP-driven annealing helicase, HepA-related protein, hHARP, SMARCA-like protein 1, Sucrose nonfermenting protein 2-like 1) is encoded by the SMARCAL1 (also known as HARP, HHARP) gene (Gene ID 50485) in human. The annealing helicase SMARCAL1 is a replication stress response protein that maintains genome integrity at stalled replication forks during DNA replication. SMARCAL1 binds Replication protein A (RPA) via its RPA-binding motif, which is both necessary and sufficient to target SMARCAL1 to stalled replication forks. Although not necessary for SMARCAL1 enzymatic activity, RPA binding is critical for its cellular function. Ataxia-telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) phosphorylate SMARCAL1 in response to replication stress. SMARCAL1-deficient cells are hypersensitive to replication stress agents and SMARCAL1 gene mutations cause Schimke immunoosseous dysplasia (SIOD).

Specificità

Reacts with both human and murine SMARCAL1. Expected to react with all four spliced isoforms of murine SMARCAL1.

Immunogeno

Recombinant protein corresponding to the N-terminus of human SMARCAL1.

Applicazioni

Anti-SMARCAL1 Antibody, Cat. No. ABE1836, is a highly specific rabbit Polyclonal antibody, that targets SMARCAL1 and has been tested in Western Blotting, Immunoprecipitation.

Immunoprecipitation Analysis: 3.0 µL from a representative lot immunoprecipitated SMARCAL1 in HEK293T cell lysate (Data courtesy of Dr. David Cortez, Vanderbilt University).
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected SMARCAL1 in siRNA transfected HEK293T cell lysate (Data courtesy of Dr. David Cortez, Vanderbilt University).
Western Blotting Analysis: A representative lot detected SMARCAL1 in U2OS cell lysate (Bansbach, C.E., et al. (2009). Genes Dev. 23(20):2405-2414).

Qualità

Evaluated by Western Blotting in mouse myoblast C2C12 nuclear extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected SMARCAL1 in 10 µg of mouse myoblast C2C12 nuclear extract.

Descrizione del bersaglio

~110 kDa observed. Human SMARCAL1 (105.9 kDa calculated). Mouse SMARCAL1 (100.8 kDa/isoform 1, 87.1 kDa/isoform 2, 63.2 kDa/isoform 3, 44.2 kDa/isoform 4 calculated). Uncharacterized band(s) may appear in some lysates.

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1