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AB4330

Sigma-Aldrich

Anti-DDX4 (Vasa) Antibody

from rabbit, purified by affinity chromatography

Sinonimo/i:

DEAD (Asp-Glu-Ala-Asp) box polypeptide 4, probable ATP-dependent RNA helicase DDX4, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4, Vasa homolog, DEAD box protein 4

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Purificato mediante

affinity chromatography

Reattività contro le specie

rat, human, mouse

tecniche

immunohistochemistry: suitable (paraffin)
western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... DDX4(54514)

Descrizione generale

DDX4 (Vasa or MVH) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. DDX4 (Vasa) proteins are mostly localized in testicular germ cells in the cytoplasm of spermatocytes. DDX4 (Vasa) is a high-copy extragenic suppressor gene. DDX4 (Vasa) is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility.

Specificità

This antibody recognizes DDX4 (Vasa) at the C-terminus.

Immunogeno

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of mouse DDX4 (Vasa).

Applicazioni

Anti-DDX4 (Vasa) Antibody detects level of DDX4 (Vasa) & has been published & validated for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected DDX4 (Vasa) in normal human testis tissue.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

RNA Metabolism & Binding Proteins

Qualità

Evaluated by Western Blot in mouse testis tissue lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected DDX4 (Vasa) in 10 µg of mouse testis tissue lysate. Western Blot performed without Tween.

Descrizione del bersaglio

~76 kDa observed

Stato fisico

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Mouse testis tissue lysate

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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A population of small stem cells with diameters of up to 5 μm resembling very small embryonic-like stem cells (VSELs) were sorted from human embryonic stem cell (hESC) cultures using magnetic-activated cell sorting (MACS) based on the expression of a
Tzu-Bou Hsieh et al.
Journal of ovarian research, 17(1), 37-37 (2024-02-10)
Premature ovarian insufficiency (POI) is a condition defined as women developing menopause before 40 years old. These patients display low ovarian reserve at young age and difficulties to conceive even with assisted reproductive technology. The pathogenesis of ovarian insufficiency is

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