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AB2999

Sigma-Aldrich

Anti-BNIP3 Antibody

from rabbit, purified by affinity chromatography

Sinonimo/i:

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
application:
ICC
WB
Reattività contro le specie:
human
tecniche:
immunocytochemistry: suitable
western blot: suitable
citations:
2

Origine biologica

rabbit

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Purificato mediante

affinity chromatography

Reattività contro le specie

human

Reattività contro le specie (prevista in base all’omologia)

mouse (based on 100% sequence homology)

tecniche

immunocytochemistry: suitable
western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... BNIP3(664)

Descrizione generale

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3) is a member of the NIP3 family, a singular group of death-inducing, mitochondrial proteins. BNIP3 is comprised of several domains of interest; a highly conserved domain (CD), a C-terminal transmembrane (TM) domain, and a BH3 domain. Strongly upregulated by hypoxia, BNIP3 induces a caspase-independent cell death characterized by mitochondrial damage. Overexpression has been observed in the brain after stroke and in the adult heart following myocardial infarction. Recent studies have shown BNIP3 to be involved in cell growth inhibition through suppression of the mTOR pathway and that, when localized to the nucleus, it has the paradoxical capability of repressing apoptosis in tumors by binding the AIF expression gene.

Specificità

Other homologies: Rat, equine, and ox (90% sequence homology).

Immunogeno

Epitope: Internal (C-terminal half).
KLH-conjugated linear peptide corresponding to an internal sequence within the C-terminal half of human BNIP3.

Applicazioni

Anti-BNIP3 Antibody detects level of BNIP3 & has been validated for use in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected hypoxia-induced BNIP3 upregulation in human glioblastoma-astrocytoma, epithelial-like U87 cells (Courtesy of Spencer Gibson, Manitoba Institute of Cell Biology).
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Qualità

Evaluated by Western Blotting in PC3 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected BNIP3 in 10 µg of PC3 cell lysate.

Descrizione del bersaglio

~60 kDa (dimer) observed. BNIP3 may be observed as a 30 kDa monomer and/or a 60 kDa dimer.

Stato fisico

Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
PC3 cell lysate.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Y Yuan et al.
Cell death & disease, 4, e690-e690 (2013-06-29)
The histone methyltransferase G9a is overexpressed in a variety of cancer types, including pancreatic adenocarcinoma, and promotes tumor invasiveness and metastasis. We recently reported the discovery of BRD4770, a small-molecule inhibitor of G9a that induces senescence in PANC-1 cells. We
Lisa A Brennan et al.
Experimental eye research, 174, 173-184 (2018-06-08)
The formation and life-long growth of the ocular lens depends on the continuous differentiation of lens epithelial cells into lens fiber cells. To achieve their mature structure and transparent function, newly formed lens fiber cells undergo a series of cellular

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