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Key Documents

AB10545

Sigma-Aldrich

Anti-TIGAR Antibody

from rabbit, purified by affinity chromatography

Sinonimo/i:

chromosome 12 open reading frame 5, TP53-induced glycolysis and apoptosis regulator, transactivated by NS3TP2 protein, probable fructose-2,6-bisphosphatase TIGAR

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

100

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Purificato mediante

affinity chromatography

Reattività contro le specie

mouse, human, rat

Reattività contro le specie (prevista in base all’omologia)

bovine (based on 100% sequence homology)

tecniche

immunohistochemistry: suitable (paraffin)
western blot: suitable

N° accesso NCBI

NP_065108

N° accesso UniProt

Q9NQ88

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... TIGAR(57103)

Categorie correlate

Descrizione generale

TIGAR (TP53-induced glycolysis and apoptosis regulator) is a p53-inducible protein that is thought to diminish levels of reactive oxygen species (ROS) and this may also contribute to p53 protecting from genomic damage. TIGAR’s ability to lower ROS levels may also have an important role to play in the autophagic response. It is known to decrease levels of fructose-2,6-bisphosphate and in turn inhibits glycolysis. Under ischemic stress, recent research has shown that TIGAR and p53 can regulate cellular energy homeostasis and apoptosis.

Immunogeno

KLH-conjugated linear peptide corresponding to human TIGAR.

Applicazioni

Anti-TIGAR Antibody is an antibody against TIGAR for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:400 dilution from a previous lot detected TIGAR in normal human brain and glioblastoma cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Qualità

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected TIGAR in 10 µg of HeLa cell lysate.

Descrizione del bersaglio

~30 kDa observed

Stato fisico

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
HeLa cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Eric C Cheung et al.
Cancer cell, 37(2), 168-182 (2020-01-28)
The TIGAR protein has antioxidant activity that supports intestinal tissue repair and adenoma development. Using a pancreatic ductal adenocarcinoma (PDAC) model, we show that reactive oxygen species (ROS) regulation by TIGAR supports premalignant tumor initiation while restricting metastasis. Increased ROS
Mark D Wilkie et al.
Cancer letters, 478, 107-121 (2020-03-03)
Patients with mutated TP53 have been identified as having comparatively poor outcomes compared to those retaining wild-type p53 in many cancers, including squamous cell carcinomas of the head and neck (SCCHN). We have examined the role of p53 in regulation
Yan Tang et al.
eLife, 11 (2022-03-08)
Cholinergic and sympathetic counter-regulatory networks control numerous physiological functions, including learning/memory/cognition, stress responsiveness, blood pressure, heart rate, and energy balance. As neurons primarily utilize glucose as their primary metabolic energy source, we generated mice with increased glycolysis in cholinergic neurons

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