567693

SOST, Mouse, Recombinant

• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Saggio: ≥90% (SDS-PAGE)
• Livello qualitativo: 100
• Forma fisica: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Impurezze: <1.0 EU/μg protein Endotoxin
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Recombinant, mouse sclerostin (SOST) consisting of amino acids 24-211, expressed in NS0-derived murine myeloma cells with an N-terminal His•Tag. SOST is expressed by osteoclasts in developing bones of mouse embryos and acts as a negative regulator of bone formation through repression of BMP-induced osteogenesis.

Azioni biochim/fisiol

Inhibition Assay: Inhibits hBMP-6-induced alkaline phosphatase by 50-80% in MC3T3-E1 mouse osteoblastic cells when used at a concentration of 30 µg/ml.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

Lyophilized from 0.2 µm filter-sterilized PBS, BSA.

Ricostituzione

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 200 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Altre note

Kusu, N. et al. 2003. J. Biol. Chem.278, 24113.
Balemans, W. et al. 2001. Hum. Mol. Genet.10 537.
Brunkow, M.E. et al. 2001. Am. J. Hum. Genet.68, 577.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3