382159-M

PhosphoDetect Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb

liquid, Calbiochem^®

• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: liquid
• contiene: ≤0.1% sodium azide as preservative
• Reattività contro le specie: Xenopus, Tetrahymena sp., human, Drosophila, Caenorhabditis elegans
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• Isotipo: IgG
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: phosphorylation (pSer10)
• Informazioni sul gene: human ... H3C1(8350)

Descrizione

Descrizione generale

Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser¹⁰.

Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser¹⁰.

This PhosphoDetect Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb is validated for use in Immunoblotting, ICC, IHC, IP for the detection of Histone H3 (pSer¹⁰) (7-20).

Immunogeno

Human

a synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH

Applicazioni

Immunoblotting (0.5 µg/ml)

Immunocytochemistry (5 µg/ml)

Immunohistochemistry (see comments)

Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C).

Risultati analitici

Positive Control
Acid-extracted proteins from colcemid-arrested HeLa cells

Altre note

Ajiro, K., et al. 1996. J. Biol. Chem.271, 13197.
Chadee, D.N., et al. 1995. J. Biol. Chem.270, 20098.
Mahadevan, L.C., et al. 1991. Cell65, 775.

For immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable