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16-232

Sigma-Aldrich

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 488 conjugate

clone DM1A, Upstate®, from mouse

Sinonimo/i:

Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain, tubulin, alpha 1a, tubulin, alpha 3, tubulin, alpha, brain-specific

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

100

Coniugato

ALEXA FLUOR™ 488

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

DM1A, monoclonal

Reattività contro le specie

rat, mouse, guinea pig, human, pig, bovine, avian

Produttore/marchio commerciale

Upstate®

tecniche

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

Isotipo

IgG1

N° accesso NCBI

NP_006000

N° accesso UniProt

Q71U36

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... TUBA1A(7846)

Descrizione generale

Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.

Specificità

Recognizes α-Tubulin, clone DM1A, Mr 50-60 kDa.

Immunogeno

Epitope: The epitopehas been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).
Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).

Applicazioni

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate is an antibody against α-Tubulin for use in FC, IC, IF & WB.
Western Blot Analysis:
2 µg/mL of a previous lot detected α-Tubulin in RIPA lysates from A431 cells.

Immunocytochemistry:
2 µg/mL of a previous lot showed positive immunostaining for α-Tubulin in HeLa cells.

Qualità

Routinely evaluated by Flow Cytometry.

Flow Cytometry:
0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6, Jurkat, and A431 cells.

Descrizione del bersaglio

50-60 kDa

Stato fisico

Alexa Fluor™ 488 conjugated, purified mouse monoclonal IgG1 in buffer containing PBS containing 1% BSA, 0.05% Tween-20, 0.05% sodium azide.
Liquid at 2-8°C.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.
Do Not Freeze. Do not store the material diluted.
For maximum recovery of product, centrifuge original vial prior to removing cap.

Risultati analitici

Control
Negative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor™ 488-conjugate.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Simon Schneider et al.
eLife, 12 (2023-11-28)
Cylicins are testis-specific proteins, which are exclusively expressed during spermiogenesis. In mice and humans, two Cylicins, the gonosomal X-linked Cylicin 1 (Cylc1/CYLC1) and the autosomal Cylicin 2 (Cylc2/CYLC2) genes, have been identified. Cylicins are cytoskeletal proteins with an overall positive
Yin-Wei Kuo et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(12), 5533-5541 (2019-03-07)
The remodeling of the microtubule cytoskeleton underlies dynamic cellular processes, such as mitosis, ciliogenesis, and neuronal morphogenesis. An important class of microtubule remodelers comprises the severases-spastin, katanin, and fidgetin-which cut microtubules into shorter fragments. While severing activity might be expected
Naila Umer et al.
Frontiers in cell and developmental biology, 9, 749559-749559 (2021-12-07)
Profilins (PFNs) are key regulatory proteins for the actin polymerization in cells and are encoded in mouse and humans by four Pfn genes. PFNs are involved in cell mobility, cell growth, neurogenesis, and metastasis of tumor cells. The testes-specific PFN3
Wei-Ting Yueh et al.
Development (Cambridge, England) (2021-12-15)
Aneuploidy is frequently observed in oocytes and early embryos, begging the question of how genome integrity is monitored and preserved during this critical period. SMC3 is a subunit of the cohesin complex that supports genome integrity, but its role in
Crystallographic structure of tubulin: implications for dynamics and drug binding.
Downing, K H and Nogales, E
Cell Structure and Function, 24, 269-275 (1999)
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