14-773-M

BTK (E41K) Protein, active, 10 µg

• Codice UNSPSC: 12352204
• eCl@ss: 32160405
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Livello qualitativo: 100; 300
• PM: M_w 78.4 kDa
• Produttore/marchio commerciale: Upstate^®
• tecniche: activity assay: suitable (kinase)
• N° accesso NCBI: NM_000061
• N° accesso UniProt: P35991 (mouse); Q06187

Descrizione

Descrizione generale

Expressed by baculovirus in Sf21 insect cells

N-terminal His6-tagged, recombinant, full-length, human BTK

Specific mutations within the pleckstrin homology (PH) domain of BTK results in human X-linked agammaglobulinemia (XLA). The E41K substitution, identified through random mutagenesis, is a PH domain gain-of-function mutation that has been shown to be associated with increased membrane localization and tyrosine phosphorylation of BTK. Using animal models, this mutation has been shown to arrest the development of immature B cells and to act as a tumour suppressor in B-cell linker protein (BLNK/SLP-65)-deficient hosts. (Li, .et al., Immunity,(1995);2:451-460 and Baraldi, E., et al., Structure,(1999);7:449-460).

Applicazioni

Active, N-terminal His6-tagged, recombinant, full-length, human BTK, for use in Kinase Assays.

Research Category
Inflammation & Immunology

Azioni biochim/fisiol

• Protein Target: BTK
• Target Sub-Family:TK

Confezionamento

Also available in 250 μg size --call for pricing and availability and reference catalog number 14-773M when ordering the 250 μg size.

Qualità

Routinely evaluated by phosphorylation of LKBtide.

Stato fisico

Format: Ni²⁺/NTA-agarose

Stoccaggio e stabilità

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediat

Altre note

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H317
• Consigli di prudenza: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classi di pericolo: Skin Sens. 1
• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable