07-848-I

Anti-phospho-IRS1 Antibody (Tyr941)

from rabbit, purified by affinity chromatography

• Sinonimo/i: Insulin receptor substrate 1, Tyr941 phosphorylated, IRS-1, Tyr941 phosphorylated
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• tecniche: western blot: suitable
• N° accesso NCBI: NP_005535
• N° accesso UniProt: P35568
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: phosphorylation (pTyr941)
• Informazioni sul gene: human ... IRS1(3667)

Descrizione

Descrizione generale

Insulin receptor substrate 1 (UniProt P35568; also known as IRS-1) is encoded by the IRS1 gene (Gene ID 3667) in human. The insulin receptor substrates (IRS-1, IRS-2, IRS-3, and IRS-4) are adaptor proteins involved in modulating cell growth, metabolism, survival, and differentiation by transducing receptor signaling to multiple downstream effectors. IRS family proteins contain a conserved N-terminal pleckstrin homology (PH) domain (a.a. 12-115 of human IRS-1), a phosphotyrosine-binding (PTB) domain (a.a. 160-264 of human IRS-1), and multiple tyrosine phosphorylation sites in the C-terminal region, including Tyr612, Tyr896, Tyr941, Tyr1158, and Tyr1220. The PTB domain meidates the interaction of IRS-1, -2 and -3 with IR juxtamembrane NPXYp motif, facilitating subsequent receptor-mediated IRS tyrosine phosphorylation. Upon phosphorylation, IRS proteins serve as binding sites for Src homology 2 (SH2) domain-containing proteins, such as SHP-2 and PI 3-kinase. Functions of IRS proteins are largely dependent on the phosphorylations of specific tyrosine residues, which in turn are subject to negative regulation by phosphatase activity. In addition, IRS proteins can be phosphorylated by serine/threonine kinases, such as PI-3 kinase, Akt/PKB, GSK-3, mTOR, and PKC, and increased Ser/Thr phosphorylation reduces their binding to IR and tyrosine phosphorylation. Inhibition of PI 3-kinase enhances insulin-stimulated IRS-1 Tyr612/Tyr941 phosphorylation (p85-binding sites) and increases IRS-1 association with PI 3-kinase p85 subunit.

Specificità

This polyclonal antibody detected a ~165 kDa target band in lysates from serum-starved HEK293 cells only after, but not before, insulin stimulation. Target region sequence is not 100% conserved among human, mouse, and rat species.

Immunogeno

KLH-conjugated phosphopeptide corresponding to human IRS1 target region sequence containing phosphorylated Tyr941.

Applicazioni

Detect IRS1 Tyr941 phosphorylation using this rabbit polyclonal Anti-phospho-IRS1 (Tyr941) antibody, Cat. No. 07-848-I, validated for use in Western Blotting.

Research Category
Signaling

Qualità

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:200 dilution of this antibody detected IRS1 Tyr941 phosphorylation induction in 10 µg of lysate from serum-starved HEK293 cells following insulin treatment.

Descrizione del bersaglio

~165 kDa observed. 131.6 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: 07-848

Stato fisico

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable