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Documenti fondamentali

07-030-AF647

Sigma-Aldrich

Anti-dimethyl Histone H3 Antibody (Lys4), Alexa Fluor 647 Conjugate

from rabbit

Sinonimo/i:

H3K4me2, H3.3B, Lys4 dimethylated, H3 histone, family 3A, Lys4 dimethylated, H3 histone, family 3B, Lys4 dimethylated, Histone H3, Lys4 dimethylated

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702

Origine biologica

rabbit

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Reattività contro le specie

human, Tetrahymena sp.

tecniche

immunocytochemistry: suitable

N° accesso NCBI

N° accesso UniProt

modifica post-traduzionali bersaglio

dimethylation (Lys4)

Informazioni sul gene

human ... HIST1H3F(8968)

Descrizione generale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

Specificità

Broad species cross-reactivity is expected.
Specificity of this polyclonal antibody toward histone H3 Lys4 dimethylation was demonstrated by dot blot analysis using the unconjugated antiserum (Cat. No. 07-030).

Immunogeno

Epitope: H3K4me2
KLH-conjugated synthetic peptide (ART[Kme2]QTAR-GC) corresponding to amino acids 1-8 of human histone H3.

Applicazioni

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
The unconjugated antiserum (Cat. No. 07-030) is shown to be suitable also for dot blot, chromatin immunoprecipitation (ChIP), ChIP-seq, immunocytochemistry, immunofluorescence, and Western blotting applications.
This Anti-dimethyl Histone H3 Antibody (Lys4), Alexa Fluor 647 Conjugate is validated for use in Immunocytochemistry for the detection of dimethyl Histone H3.

Qualità

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclear histone H3 Lys4 dimethylation in A431 cells.

Descrizione del bersaglio

~17 kDa observed.

Stato fisico

Format: Purified
Protein A purified.
Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Note legali

ALEXA FLUOR is a trademark of Life Technologies

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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