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917834

Sigma-Aldrich

PhotoCol-RUT, methacrylated collagen bioink kit, with ruthenium

Sinonimo/i:

3D Bioprinting, Bioink, Collagen

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About This Item

Codice UNSPSC:
12352201
NACRES:
NA.23

Descrizione

Methacrylated collagen:
Degree of methacrylation ≥ 20%

Product components :
Methacrylated collagen (100 mg)
20 mM acetic acid (50 mL)
Neutralization solution (10 mL)

Ruthenium (100 mg)
Sodium persulfate photoinitiator (500 mg)

Livello qualitativo

Sterilità

sterile; sterile-filtered

Impurezze

≤10 EU/mL Endotoxin

Temperatura di conservazione

2-8°C

Applicazioni

PhotoCol-RUT bioink kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents. The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the α-amines groups on the N-termini. Over 20% of the total lysine residues of the collagen molecule have been methacrylated. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture. The photoinitiator consists of ruthenium and sodium persulfate to be formulated in 1X cell culture media or PBS, which allows visible light photocrosslinking of the printed structure at 400-450 nm. PhotoCol-RUT provides native-like 3D collagen gels, and the final gel stiffness can be customized by changing collagen concentrations and crosslinking.

Note legali

PhotoCol is a trademark of Advanced BioMatrix, Inc.

Pittogrammi

Environment

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Aquatic Chronic 2

Codice della classe di stoccaggio

10 - Combustible liquids


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