P1435
Phosphatase, Acid from sweet potato
ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian)
Synonym(s):
(Orthophosphoric-monoester phosphohydrolase [acid optimum] )
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 500 units | Please contact Customer Service for Availability | €385.00 | |
| 2500 units | Please contact Customer Service for Availability | €1,710.00 |
About This Item
CAS Number:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-630-9
MDL number:
eCl@ss:
42010102
Specific activity:
≥10.0 units/mg protein (modified Warburg-Christian)
€385.00
Please contact Customer Service for Availability
For a suitable alternative that ships today, try P3752-100MG.
form
ammonium sulfate suspension
Quality Level
specific activity
≥10.0 units/mg protein (modified Warburg-Christian)
secondary activity
≤9.9 units/mg protein ATPase
mol wt
110 kDa
storage temp.
2-8°C
SMILES string
O(CC(O)C)CC#C
InChI
1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
InChI key
GZCWLCBFPRFLKL-UHFFFAOYSA-N
General description
Acid phosphatase from sweet potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar molecular mass but with different isoelectric points. [1]
Application
Potato acid phosphatase is used as a dephosphorylating reagent in thiochrome assays for the detection of thiamin in biological samples. In this assay, it was shown to be more effective than acid phosphatases from wheat germ or α-amylase. [2]
Sweet potato acid phosphatase has been used in a study as a pre-column enzyme reactor via a covalent bond with glutaraldehyde and aminopropyl controlled-pore glass. [3] It has also been used in a study to investigate the use of tyrosine, histidine, and cysteine as ligands for Mn(III) in sweet potato phosphatase.
Biochem/physiol Actions
Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7 by the following reaction: APaseR-PO4 + H2O --------------- R-OH + HOPO3 2+Their function in the production, transport, and recycling of phosphate is critical for the metabolic and energy transduction processes of the cell. As a group, Apases may be as important as kinases in regulatory processes.
Physical form
Suspension in 1.8 M (NH4)2SO4, 10 mM MgCl2, pH 5.3
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥10.0 units/mg protein (modified Warburg-Christian) | specific activity 3.0-10.0 unit/mg solid | specific activity ≥3.0 units/mg solid | specific activity ≥0.5 unit/mg solid |
| form ammonium sulfate suspension | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| mol wt 110 kDa | mol wt - | mol wt 69 kDa | mol wt 69 kDa |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 300 | Quality Level 400 | Quality Level 300 | Quality Level 200 |
| secondary activity ≤9.9 units/mg protein ATPase | secondary activity - | secondary activity - | secondary activity - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Related Content
G Schenk et al.
Archives of biochemistry and biophysics, 370(2), 183-189 (1999-10-08)
Purple acid phosphatases comprise a family of binuclear metal-containing acid hydrolases, representatives of which have been found in animals, plants, and fungi. The goal of this study was to characterize purple acid phosphatases from sweet potato tubers and soybean seeds
Susumu Yamato et al.
Biological & pharmaceutical bulletin, 27(2), 210-215 (2004-02-06)
Sweet potato acid phosphatase was covalently coupled with glutaraldehyde to aminopropyl controlled-pore glass, and used as a pre-column enzyme reactor. The immobilized enzyme reactor (IMER) was continuously operated using an automated chromatographic detection system we developed. Functional evaluation of the
An unstable manganese(III) complex incorporating ligand donor types proposed for an acid phosphatase from sweet potato: (p-nitrobenzenethiolato)[N,N'-ethylenebis(salicylideneaminato)]manganese(III)
Gohdes, J. and W. Armstrong
Inorganic Chemistry, 27, 1841-1842 (1988)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P1435-2.5KU | 04061832758749 |
| P1435-500UN | 04061834354987 |