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U937 Cell Line human

85011440, human lung (lymphoblast), Lymphoblast

Synonym(s):

U-937 Cells

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About This Item

UNSPSC Code:
41106514
Pricing and availability is not currently available.

Product Name

U937 Cell Line human, from human lung(lymphoblast), 85011440

biological source

human lung (lymphoblast)

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian histiocytic lymphoma

Cell Line Description

Derived from malignant cells of a pleural effusion of 37 year old caucasian male with diffuse histiocytic lymphoma. One of only a few human lines still expressing many of the monocytic like characteristics exhibited by cells of histiocytic origin.

Application

Tumourigenicity studies
U937 Cell Line human has been used to study the toxicity of propolis extracts against U937 cells and to study the uptake of apoptotic bodies (derived from monocytic U-937 cells) by primary meningothelial cells (PMECs).[1][2]

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 10,12
D16S539: 12
D5S818: 12
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
vWA: 14,15

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C. Cells may take up to 72 hours until confluent.

Other Notes

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Articles

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

Questions

1–7 of 7 Questions  

  1. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 answer

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

      Helpful?

  2. How many Cell Line cells are in the vial?

    1 answer

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

      Helpful?

  3. Are my Cell Line cells mycoplasma tested?

    1 answer

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

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  4. What medium should I use when I thaw the Cell Line cells?

    1 answer

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

      Helpful?

  5. What passage are my Cell Line cells?

    1 answer

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

      Helpful?

  6. How do I handle Cell Line frozen cells upon arrival?

    1 answer

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

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  7. Are the Cell Line cells tested for viral pathogens?

    1 answer

    1. HPA Cultures does not perform any viral testing on the cell lines.

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