Skip to Content
Merck
All Photos(1)

Key Documents

K562 Cell line human

89121407, human blood (chronic myelogenous leukemia), Lymphoblast

Synonym(s):

K-562 Cells

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41106514
Pricing and availability is not currently available.

Product Name

K562 Cell line human, 89121407, from human blood(chronic myelogenous leukemia)

biological source

human blood (chronic myelogenous leukemia)

growth mode

Suspension

karyotype

2n = 46

morphology

Lymphoblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian chronic myelogenous leukaemia

Cell Line Description

Established from pleural effusion of 53 year old female with chronic myelogenous leukaemia in terminal blast crisis. Population highly undifferentiated and of the granulocytic series. Can be used as a highly sensitive target for the in vitro natural killer assay. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series.

Application

K562 cell line has been used to study the protecting role of cluster of differentiation-39 (CD39) attenuation against DNA damage. It has also been used to study the relevance of a novel sorting approach for separating cells, called “Slice and Dice Sorting”.[1][2]
Natural killer assay and tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 8
D16S539: 11,12
D5S818: 11,12
D7S820: 9,11
THO1: 9.3
TPOX: 8,9
vWA: 16

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

1–9 of 9 Questions  

  1. Do K562 cells express CD34?

    1 answer

    1. The K562 cell line isn't tested for CD markers. This cell line is a product of ECACC and were originally obtained from ATCC. According to various publications these cells do express CD34. See the link below for one of these publications:
      https://plos.figshare.com/articles/figure/Flow_cytometry_of_cell_surface_markers_of_K562_and_K562-IR_cells_/11909724/1

      See the link below for the ECACC product page:
      https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=89121407&collection=ecacc_gc#medDoc

      Helpful?

  2. Is the BCR/ABL cell gene positive?

    1 answer

    1. This cell line is a banked product of ECACC. As stated on the ECACC product page, the line was acquired from ATCC. The original depositor listed at ATCC is HT Holden and appears to have been established sometime in the 1970s. There is no confirmation of the Bcr-Abl by either supplier. However, see the link below for a reference that appears to suggest that this line is Bcr-Abl positive. This publication has not been validated.
      https://pubmed.ncbi.nlm.nih.gov/16465215/

      For more technical information, please visit the website with this product page: https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=89121407&collection=ecacc_gc

      Helpful?

  3. How many Cell Line cells are in the vial?

    1 answer

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

      Helpful?

  4. Are the Cell Line cells tested for viral pathogens?

    1 answer

    1. HPA Cultures does not perform any viral testing on the cell lines.

      Helpful?

  5. Are my Cell Line cells mycoplasma tested?

    1 answer

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

      Helpful?

  6. How do I handle Cell Line frozen cells upon arrival?

    1 answer

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

      Helpful?

  7. What passage are my Cell Line cells?

    1 answer

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

      Helpful?

  8. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 answer

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

      Helpful?

  9. What medium should I use when I thaw the Cell Line cells?

    1 answer

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service