MM537
12070509, human skin, Epithelial
Synonym(s):
MM 537
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About This Item
UNSPSC Code:
41106514
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product name
MM537, 12070509
biological source
human skin
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
receptors
Not specified
relevant disease(s)
metastasis
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Cell Line Origin
Human melanoma; from metastatic site - lymph node
Cell Line Description
mM537 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1356.
DNA Profile
Not specified
Culture Medium
RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + 10% FBS.
Subculture Routine
Optimal split ratio 1:4 (minimum seeding density 2.7 x 104 cells/cm2). Cells incubated at 37 °C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 1-2 min. MM537 required growth medium to be changed 3 times each week. The cells are sensitive to trypsin so exposure time to trypsin should be kept to a minimum. The cells take 2-3 days to recover from trypsinisation.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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