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Key Documents

WBLUR

Millipore

Immobilon® Crescendo Western HRP substrate

Western blotting, dot/slot blotting

Synonym(s):

HRP substrate, chemiluminescent detection substrate, detection substrate

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About This Item

UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22

product name

Immobilon Crescendo Western HRP substrate, 100 mL, Western blotting, dot/slot blotting

conjugate

peroxidase conjugate

Quality Level

200

packaging

pkg of 100 mL

manufacturer/tradename

Luminata

technique(s)

western blot: suitable

membrane area

1000 cm2 , membrane coverage

detection method

chemiluminescent

shipped in

ambient

General description

Premixed, ready to use chemiluminescent HRP detection reagent

Application

For use on immunoblots.
Western blotting, dot/slot blotting

Packaging

1 bottle for 1000 cm2

Quality

The reagent has been matched to optimize the range and sensitivity of detection using various antibodies in Western blot assays.

Storage and Stability

Stable for 1 year at 4°C from date of shipment. Contains hydrogen peroxide. Use in well ventilated area.

Legal Information

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 2

Certificates of Analysis (COA)

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Articles

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Protocols

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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