MABE308
Anti-ICBP90/UHRF1 Antibody, clone 1RC1C-10
ascites fluid, clone 1RC-1C10, from mouse
Synonym(s):
E3 ubiquitin-protein ligase, inverted CCAAT box-binding protein, Nuclear protein 95, Nuclear zinc finger protein Np95, HuNp95, RING finger protein 106, Transcription factor ICBP90, Ubiquitin-like PHD and RING finger domain-containing protein 1, Ubiquitin
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
1RC-1C10, monoclonal
species reactivity
human
technique(s)
ChIP: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... UHRF1(29128)
General description
Ubiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers.
Immunogen
Recombinant protein corresponding to human ICBP90.
Application
Anti-ICBP90/UHRF1 Antibody, clone 1RC1C-10 is a Mouse Monoclonal Antibody for detection of ICBP90/UHRF1 also known as E3 ubiquitin-protein ligase, inverted CCAAT box-binding protein & has been validated in WB, ChIP, ICC & IHC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Western Blot Analysis: A representative lot from an independent laboratory detected ICBP90/UHRF1 in HeLa and MOLT-4 cell lysates (Hopfner, R., et al. (2001). 266(1-2)15-23.).
Immunocytochemistry Analyisis: A representative lot from an independent laboratory detected ICBP90/UHRF1 in ICC (Hopfner, R., et al. (2001). Gene. 266(1-2)15-23.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected ICBP90/UHRF1 in human appendix and human breast carcinoma tissues (Hopfner, R., et al. (2000). Cancer Res. 60(1):121-128.).
Chromatin Immunoprecipitation Analyis: A representative lot from an independent laboratory immunoprecipitated ICB90/UHRF1 in ChIP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19). 8355-8365.)
Immunocytochemistry Analyisis: A representative lot from an independent laboratory detected ICBP90/UHRF1 in ICC (Hopfner, R., et al. (2001). Gene. 266(1-2)15-23.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected ICBP90/UHRF1 in human appendix and human breast carcinoma tissues (Hopfner, R., et al. (2000). Cancer Res. 60(1):121-128.).
Chromatin Immunoprecipitation Analyis: A representative lot from an independent laboratory immunoprecipitated ICB90/UHRF1 in ChIP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19). 8355-8365.)
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected ICBP90/UHRF1 in 10 µg of HeLa cell lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected ICBP90/UHRF1 in 10 µg of HeLa cell lysate.
Target description
~90 kDa observed
Physical form
Mouse monoclonal IgG1 ascites with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Michael D Rushton et al.
Epigenetics, 17(12), 1590-1607 (2022-03-25)
Propagation of DNA methylation through cell division relies on the recognition of methylated cytosines by UHRF1. In reprogramming of mouse embryonic stem cells to naive pluripotency (also known as ground state), despite high levels of Uhrf1 transcript, the protein is
Yusuke Murase et al.
The EMBO journal, 39(21), e104929-e104929 (2020-09-22)
Human germ cells perpetuate human genetic and epigenetic information. However, the underlying mechanism remains elusive, due to a lack of appropriate experimental systems. Here, we show that human primordial germ cell-like cells (hPGCLCs) derived from human-induced pluripotent stem cells (hiPSCs)
Roxane Verdikt et al.
EBioMedicine, 79, 103985-103985 (2022-04-17)
The multiplicity, heterogeneity, and dynamic nature of human immunodeficiency virus type-1 (HIV-1) latency mechanisms are reflected in the current lack of functional cure for HIV-1. Accordingly, all classes of latency-reversing agents (LRAs) have been reported to present variable ex vivo
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