We have not conducted specific testing to determine the assay's specificity to the active form of FGF-21. However, given that the antibodies used are polyclonal, it is likely that the assay detects Total FGF-21.
EZHFGF21
Human FGF-21 ELISA Kit
measures FGF-21 in 50 μL serum, plasma or cell culture supernatent
Synonym(s):
ELISA Kit for FGF-21, FGF-21 ELISA, Human FGF-21 Detection Kit
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96 WELLS
₹1,03,420.00
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96 WELLS
₹1,03,420.00
About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32
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Product Name
Human FGF-21 ELISA Kit, This Human FGF-21 ELISA Kit is used to measure & quantify FGF-21 levels in Metabolism & Inflammation & Immunology research.
Quality Level
species reactivity
human
packaging
kit of 1 × 96 wells
parameter
50 μL sample volume (4hr assay)
assay range
accuracy: 83-99%
standard curve range: 31.3-2000 pg/mL
inter-assay cv: <9%
intra-assay cv: <6%
technique(s)
ELISA: suitable
input
sample type serum
sample type plasma (K2 EDTA)
sample type cell culture supernatant
application(s)
research use
detection method
colorimetric (450nm/590nm)
shipped in
wet ice
storage temp.
2-8°C
General description
Recent studies have demonstrated that fibroblast growth factor 21 (FGF-21), a liver derived protein, is able to increase glucose uptake, as well as lower plasma triglyceride levels in an insulin-independent manner. FGF-21 suppresses glucagon release, thereby increasing insulin sensitivity, and reduces the amount of insulin production required by pancreatic β-cells. Unlike insulin, which works in a rapid, hormone-like manner, FGF-21 activity requires several hours, indicating changes in gene expression. While insulin functions through GLUT4 translocation, FGF-21 has been shown to act primarily through the upregulation of adipocyte GLUT1, a transporter that allows glucose to move from the blood into cells even in the absence of insulin. In addition, no significant weight gain was observed when FGF-21 was injected into animals, and mice that had been genetically engineered to overexpress FGF-21 weighed less and had less adipose tissue than the normal controls.
Specificity
No significant cross-reactivity to the cytokines and metabolic hormones tested was observed.
Application
Research Category
Metabolism
Metabolism
Research Sub Category
Diabetes - Type 2
Metabolic Syndrome
Diabetes - Type 2
Metabolic Syndrome
This Human FGF-21 ELISA Kit is used to measure & quantify FGF-21 levels in Metabolism & Inflammation & Immunology research.
This is a 3-hour assay requiring 50 μL of sample.
Used to detect/quantify: FGF-21
Other Notes
Please contact Technical Service for linearity of dilution.
Disclaimer
For research use only. Not for use in diagnostic procedures.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
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Does the Human FGF-21 ELISA (EZHFGF21-19K) detect active or total FGF-21?
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