The protocol will be on the lot-specific Certificate of Analysis.
Please see the example below:
https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB0001_0204I0225.pdf
RAB0001
Human IgG ELISA Kit
for serum, plasma
Synonym(s):
IgG, Immunoglobulin-G
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.32
Pricing and availability is not currently available.
Recommended Products
species reactivity
human
technique(s)
ELISA: suitable
capture ELISA: suitable
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Immunoglobulin G (IgG) belongs to immunoglobulin family, and consists of gamma (γ) heavy chain in the constant (C) region. IgG is a widely expressed serum antibody. Monomeric structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weights of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds linking the two heavy chains, linking the heavy and light chains, and are present inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, andγ4, respectively.[1] IgG is produced and released from CD5+ B cells, the predominant lymphocytes in the neonatal cell repertoire.[2] Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants.[3]
This ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of a target protein in biological samples such as serum, plasma, cell culture supernatants, urine and/or cell and tissue lysates. This assay employs a specific capture antibody coated on a 96 well plate.
Immunogen
Recombinant Human IGG
Application
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Storage Class Code
8B - Non-combustible corrosive hazardous materials
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Is there any specific description of the kit RAB0001? We would like to have s protocol do that we can test the IgG level in the patient sera.
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