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CRISPR12

CRISPR-Lenti Non-Targeting Control Plasmid

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About This Item

NACRES:
NA.51
UNSPSC Code:
41106609

₪1,006.00

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form

liquid

Quality Segment

packaging

vial of 50 μL

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

application(s)

CRISPR
CRISPR

shipped in

dry ice

storage temp.

−20°C

General description

The Sigma lenti CRISPR Non-Targeting Control is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. This vector is useful as a negative control in experiments using Sigma CRISPR lentiviral clones. The Sigma lenti CRISPR non-targeting control uses a one-plasmid system consisting of a EF1-alpha-driven Cas9, a U6-driven guide RNA (non-targeting), with both Puromycin and GFP co-expressed with Cas9.

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Also, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP001).

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas), originally found in bacteria and archaea, is employed by organisms to protect them from invading viruses and plasmids.

Application

Functional Genomics/Screening/Target Validation
  • Non-targeting control, CRISPR lentivirus DNA format, for creating gene knockouts/genetic modifications in cell lines.

To see more application data, protocols, vector maps visit sigma.com/crisprs.

Features and Benefits

Serves as a non-targeting or negative experimental control (DNA format) for the CRISPR editing workflow using lenti CRISPRs. This allows you to examine the effect of transfection in your system with the Sigma lenti CRISPR/Cas9 plasmid.

Physical form

The Sigma CRISPR Lenti Non-Targeting Control Vector is provided as 1 μg of plasmid DNA in Tris-EDTA (TE).

Legal Information

CRISPR Use License Agreement

Lentiviral and WPRE License Agreements

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This Item
CRISPR19CRISPR18VSHC216
CRISPR-Lenti Non-Targeting Control Plasmid

Sigma-Aldrich

CRISPR12

CRISPR-Lenti Non-Targeting Control Plasmid

Lenti CRISPR Universal Non-Target Control#2 Plasmid DNA (LV04 vector)

Sigma-Aldrich

CRISPR19

Lenti CRISPR Universal Non-Target Control#2 Plasmid DNA (LV04 vector)

Lenti CRISPR Universal Non-Target Control#1 Transduction Particles (LV04 vector)

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CRISPR18V

Lenti CRISPR Universal Non-Target Control#1 Transduction Particles (LV04 vector)

MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA Targets no known genes from any species

Sigma-Aldrich

SHC216

MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA

description

-

description

-

description

-

description

Targets no known genes from any species

form

liquid

form

liquid

form

liquid

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

packaging

vial of 50 μL

packaging

vial of 50 μL

packaging

vial of 200 μL

packaging

-

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA )

concentration

(1x106 TU/ml (via p24 titering assay))

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

application(s)

CRISPR

application(s)

CRISPR

application(s)

CRISPR

application(s)

-

Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
06111913MN
02052116MN

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