56500
GenElute™ Agarose Spin Columns
sufficient for 70 purifications
Synonym(s):
DNA purification from agarose gel, Gen Elute
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About This Item
NACRES:
NA.52
UNSPSC Code:
41106300
usage
sufficient for 70 purifications
General description
GenElute Agarose Spin Columns allow for the purification of linear DNA from agarose gels. The typical recovery is 40 to 45% for 100 bp to 10 kb DNA fragments. The recovery decreases as the fragment size increases.
The DNA band is excised from an agarose gel and loaded into the spin column. Embedded within the base of the column are a series of membranes and filters that hold agarose and impurities back while allowing DNA to selectively pass through the column into a collection tube during a 10 minute centrifugation. GenElute Agarose Spin Columns eliminate the need for silica-based resins, DEAE or toxic organic solvents such as phenol and chloroform. There is no melting, electroelution or enzymatic digestion of the agarose gel.
The purified DNA can be used in most downstream molecular biology applications including ligation, PCR, restriction digest, cloning, labeling and hybridization.
The DNA band is excised from an agarose gel and loaded into the spin column. Embedded within the base of the column are a series of membranes and filters that hold agarose and impurities back while allowing DNA to selectively pass through the column into a collection tube during a 10 minute centrifugation. GenElute Agarose Spin Columns eliminate the need for silica-based resins, DEAE or toxic organic solvents such as phenol and chloroform. There is no melting, electroelution or enzymatic digestion of the agarose gel.
The purified DNA can be used in most downstream molecular biology applications including ligation, PCR, restriction digest, cloning, labeling and hybridization.
Application
The purified DNA can be used in most downstream molecular biology applications including ligation, PCR, restriction digest, cloning, labeling and hybridization.
Biochem/physiol Actions
GenElute Agarose Spin Columns are designed for the rapid purification of linear DNA (100 bp to 10 kb) from agarose gels. The DNA band is excised from an agarose gel and loaded into the spin column. Embedded within the base of the column are a series of membranes and filters that hold agarose and impurities back while allowing DNA to selectively pass through the column into a collection tube during a 10 minute centrifugation. GenElute Agarose Spin Columns eliminate the need for silica-based resins, DEAE or toxic organic solvents such as phenol and chloroform. There is no melting, electroelution or enzymatic digestion of the agarose gel.
Features and Benefits
- One-step, 10 minute protocol
- No melting or digestion of agarose required
- Simpler and quicker than bind and elute or electroelution methods
- No ethanol precipitation
- Typical recovery of 40 to 45% for DNA fragments from 100 bp to 10 kb (Figs. 1 and 2)
- Typical recovery of 40 to 45% for DNA fragments from 100 bp to 10 kb
- No melting or digestion of agarose required
- One-step, 10 minute protocol
Other Notes
For additional information, please see www.sigma-aldrich.com/post-reaction.
GenElute™ Agarose Spin Columns isolate DNA by centrifugation/filtration.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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