The kit has been verified for use with CSF and cell/tissue culture supernatants. Protein normalization is not required for these sample types. If using the kit with cell or tissue lysates, the sample will need to be normalized to total protein, using either a BCA or Coomassie-based assay. The product has not been validated for use with cell/tissue lysates.
HNS1MAG-95K
MILLIPLEX® Human Neuroscience Magnetic Bead Panel 1 - Neuroscience Multiplex Assay
allows quantitative multiplex detection of multiple analytes simultaneously
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
To order a Milliplex® kit, please search for your analyte of interest.
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Quality Level
species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 107%
(DJ1)
accuracy: 108%
(a-Synuclein)
accuracy: 123%
(TG2)
standard curve range: 12-50,000 pg/mL
(GFAP)
standard curve range: 15-60,000 pg/mL
(NSE)
standard curve range: 20-80,000 pg/mL
(UCHL1)
standard curve range: 244-1,000,000 pg/mL
(alpha-synuclein)
standard curve range: 61-250,000 pg/mL
(DJ1)
standard curve range: 61-250,000 pg/mL
(TG2)
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
ambient
General description
Neurological dysfunction may be initiated by a broad spectrum of diseases, as well as physical injury to the brain or spinal cord. Numerous potential biomarkers of neurological dysfunction have been identified. The ability to perform multiplex analysis of these biomarkers offers a valuable research tool to better understand this area of neuroscience.
MILLIPLEX® Human Neuroscience Bead Panel 1 is a 6-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cerebrospinal fluid (CSF) samples: α-Synuclein, DJ1/PARK7, Glial fibrillary acidic protein (GFAP), Neuron specific enolase (NSE), Transglutaminase 2 (TGM2), and Ubiquitin carboxyl-terminal esterase L1 (UCHL1/PARK5).
Panel Type: Neuroscience
MILLIPLEX® Human Neuroscience Bead Panel 1 is a 6-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cerebrospinal fluid (CSF) samples: α-Synuclein, DJ1/PARK7, Glial fibrillary acidic protein (GFAP), Neuron specific enolase (NSE), Transglutaminase 2 (TGM2), and Ubiquitin carboxyl-terminal esterase L1 (UCHL1/PARK5).
Panel Type: Neuroscience
Specificity
Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Application
- Analytes: GFAP, NSE, α-Synuclein, PARK5, Transglutaminase 2 (TGM2)
- Recommended Sample type: CSF
- Recommended Sample dilution: Neat
- Assay Run Time: Overnight or one day
- Research Category: Neuroscience
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Packaging
Everything you need in a single kit.
Storage and Stability
Recommended storage for kit components is 2 - 8°C.
Other Notes
Sensitivity: See protocol for sensitivities of individual analytes
Legal Information
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Daniela Diaz-Lucena et al.
Journal of neurology, 267(9), 2567-2581 (2020-05-07)
Differential diagnosis of neurodegenerative dementia is currently supported by biomarkers including cerebrospinal fluid (CSF) tests. Among them, CSF total-tau (t-tau), phosphorylated tau (p-tau) and β-amyloid42 (Aβ42) are considered core biomarkers of neurodegeneration. In the present work, we hypothesize that simultaneous
Martin Trapecar et al.
Science advances, 7(5) (2021-01-31)
Slow progress in the fight against neurodegenerative diseases (NDs) motivates an urgent need for highly controlled in vitro systems to investigate organ-organ- and organ-immune-specific interactions relevant for disease pathophysiology. Of particular interest is the gut/microbiome-liver-brain axis for parsing out how
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How can I normalize protein for this test? Just using same volume and total concentration protein are not reliable.
1 answer-
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Can serum or plasma samples be utilized in the Human Neuroscience Panel 1 kit, considering that the protocol exclusively mentions CSF?
1 answer-
The kit has not been validated with serum/plasma samples. Eight normal plasma samples were tested at a 1:4 dilution. It was found that GFAP and UCHL1 will require more sensitivity.
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