T6066

Trizma^® base

BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

• Synonym(s): 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
• Linear Formula: NH₂C(CH₂OH)₃
• CAS Number: 77-86-1
• Molecular Weight: 121.14
• Beilstein: 741883
• EC Number: 201-064-4
• MDL number: MFCD00004679
• UNSPSC Code: 12352104
• PubChem Substance ID: 24900345
• NACRES: NA.25

Properties

• grade: BioPerformance Certified
• Quality Level: 400
• Agency: USP/NF; meets EP testing specifications; meets USP testing specifications
• description: aminopeptidase substrate
• Assay: ≥99.9% (titration)
• form: crystalline powder
• storage condition: dry at room temperature
• technique(s): DNA extraction: suitable; cell culture | mammalian: suitable; protein purification: suitable
• impurities: DNase, RNase, protease, none detected; Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested; ≤0.2% water (Karl Fischer)
• loss: ≤0.5% (loss on drying, 110 °C)
• color: white
• pH: 10.0-11.5
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• solubility: water: soluble (666 mg/ml, yielding a clear colorless solution)
• absorption: ≤0.05 at 260 at 1 M; ≤0.05 at 290 at 40%; ≤0.06 at 280 at 1 M
• suitability: suitable for chromatography; suitable for immunoprecipitation; suitable for molecular biology
• application(s): clinical research; clinical testing; diagnostic assay manufacturing
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Tris base, also known as Trometamol or Trizma base, plays a crucial role in diverse research applications as a biological buffer. Widely utilized in buffer formulations like TAE and TBE buffers, its pKa of 8.1 renders it well-suited for maintaining pH within the physiological range (pH 7.0-9.0) applicable to most living organisms. Researchers, however, need to be cautious when studying proteins, as Tris may potentially interfere with the activity of certain enzymes.

This compound can be employed independently as a buffer or as part of mixed buffer formulations, including Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, among others. Notably, Tris base is pure, essentially stable, and relatively non-hygroscopic.

In laboratory settings, Tris base proves indispensable for preparing buffers compatible with biological fluids, serving as a standard pH solution, and facilitating various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it aids in studies related to cell membrane permeability and buffer preparation.

Application

Trizma has been used:

• As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells[1]
• As a component of pre-hybridization buffer in in situ hybridization[2]
• To suspend dried protein before SDS-PAGE protein characterization[3]
• As a component of lysis buffer to wash and lyse cells before immunoprecipitation[4]
• In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis[5][6]
• In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC[7]
• In capillary electrochromatography and UV analysis of tocopherols and tocotrienols[8]
• as a component of Tris/base solution in a sulforhodamine B (SRB) assay[9]

Features and Benefits

• Suitable for Cell culture, Biochemical and Molecular Biology Research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)
• Tested for the presence of Anionic and Cationic traces
• Tested for DNase, RNase, and protease

Packaging

T6066-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

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Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves